FnCas9 Protein
SIGMA/FNCAS9PROT - from Francisella novicida, recombinant, expressed in E. coli, 1X NLS
Synonym: Francisella Novicida Cas9
Product Type: Chemical
Ribonucleoprotein (RNP) complex consisting of FnCas9 and gRNA. For use with Fn SygRNA™ synthetic gRNA.
| application(s) | CRISPR |
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized powder |
| packaging | pkg of 1 kit (4 components) |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | −20°C |
| Application: | Functional Genomics/Target Validation/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology |
| Biochem/physiol Actions: | Conventional restriction enzymes such as EcoRI and HindIII can only cut specific short (6 bp) DNA sequences and are therefore limited to cloning small DNA fragments (typically ~4 kb). CRISPR-based endonculeases can cut user defined sequences of a longer length (~20 bp) making them more flexible and applicable to larger DNA fragments such as multigene bacterial operons or mammalian genomic DNA with native promoter, exon, and intron components. |
| Features and Benefits: | • Suitable for plasmid or large genomic DNA fragment cloning • Results in 4-bp 5’ overhangs. • Cuts purified plasmid DNA more efficiently than SpCas9 |
| General description: | The purified FnCas9 protein (~190 kDa) is derived from the type II CRISPR/Cas system from the bacterium Francisella novicida and has been engineered to function using three molecular components: the FnCas9 protein, a crRNA of user-defined sequence, and a tracrRNA of constant sequence. The FnCas9 endonuclease can be programmed by changing the crRNA sequence to create a site-specific double-strand break (DSB) in plasmids or purified genomic DNA. |
| Legal Information: | CRISPR Use License Agreement |
| Legal Information: | MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany |
| Other Notes: | Click here to order gRNA Enter the 20bp genomic DNA target sequence (5′ to 3′) plus the adjacent 3′ PAM site. Under special instructions, indicate "FnCas9 crRNA" Click here to order FnCas9 tracrRNA Check out our other MISSION® Cas9 Proteins at SigmaAldrich.com/CRISPRpr |
| Other Notes: | Each kit consists of: • one vial of FnCas9 recombinant protein • one vial containing 1 mL of 1 • one vial containing 1 mL of Nuclease free water with glycerol • one vial containing 1 mL of 10× Digestion Buffer |
| Packaging: | pkg of 50 μg (≥ 300 pmol) pkg of 250 μg (≥ 1500 pmol) |
| Preparation Note: | Lyophilized FnCas9 protein should be resuspended in the Reconstitution solution provided to desired concentration. Gently tap tube to completely dissolve lyophilized powder, incubate for 10 minutes on ice, and spin tube to bring material to bottom of tube. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H319 |
| Precautionary statements | P264 - P280 - P305 + P351 + P338 - P337 + P313 |
| RIDADR | NONH for all modes of transport |
| Purity | ≥95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |
| Components | FnCas9-NLS from Francisella novicida, expressed in Escherichia coli; Dilution buffer for Cas9 proteins; Reconstitution solution for Cas9 proteins; Digestion buffer 10× for FnCas9 |