SygRNA™ FnCas9 Synthetic tracrRNA
SIGMA/FNCAS9TRACR
Product Type: Chemical
SygRNA™ synthetic Francisella novicida tracrRNA complexed with SygRNA™ synthetic Francisella novicida crRNA. For use with MISSION® FnCas9 proteins in cell-free applications.
| packaging | pkg of 1 vial |
| shipped in | ambient |
| storage temp. | −20°C |
| Application: | Functional Genomics/Genome Editing/Molecular Biology/Molecular Cloning/Synthetic Biology |
| Features and Benefits: | HPLC-purified SygRNA FnCas9 tracrRNA oligo is optimized for use with target-specific Francisella novicida Cas9 crRNA and FnCas9 protein for genome editing applications, large fragment molecular cloning, and programmable restriction digestion. |
| General description: | The SygRNA synthetic RNA system recruits FnCas9 nuclease to specific double stranded DNA targets. The FnCas9 tracrRNA (trans-activating crRNA) binds to a FnCas9 crRNA (CRISPR RNA) and the resulting crRNA-tracrRNA complex guides FnCas9 protein to crRNA-specified sites in double stranded DNA. SygRNA FnCas9 synthetic tracrRNA and crRNAs are optimized for use with FnCas9 protein and compatible with a variety of nucleic acid delivery and digestion methods. To purchase custom crRNAs click "Request Quotation for Custom crRNA Synthesis" at sigma.com/SygRNA  |
| Legal Information: | CRISPR Use License Agreement |
| Legal Information: | SygRNA is a trademark of Sigma-Aldrich Co. LLC |
| Physical form: | Dry |
| Preparation Note: | Resuspend SygRNA FnCas9 tracrRNA oligo in RNase-free Tris-containing buffer with a pH 8.0 or less. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106609 |