Monoclonal Anti-Glutathione-S-Transferase (GST) antibody produced in mouse

SIGMA/G1160 - clone GST-2, ascites fluid

Synonym: Mouse Anti-GST Tag

MDL Number: MFCD00212963
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G1160-100UL	100 µL	$552.00 1/EA	Add To Favorites
45-G1160-.2ML	0.2 mL	$727.00 1/EA	Add To Favorites

Western Blotting JOURNAL CITATION: The homeodomain transcription factor Hoxa2 interacts with and promotes the proteasomal degradation of the E3 ubiquitin protein ligase RCHY1. By: Bergiers, I., Bridoux, L., et al. in PLoS One, 2013. PubMed ID: 24244684 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0080387), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

Western Blotting JOURNAL CITATION: Snapin, positive regulator of stimulation- induced Ca²⁺ release through RyR, is necessary for HIV-1 replication in T cells. By: Kinoshita, S. M., Kogure, A., et al. in PLoS One, 2013. PubMed ID: 24130701 Image collected and cropped by CiteAb from the following publication, (https://dx.plos.org/10.1371/journal.pone.0075297), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.

Immunoblotting Purified GST (G5663) was separated on SDS-PAGE and probed with Monoclonal AB TO GST CL:GST-2 (Cat. No. G1160). The antibody was developed with HRP APA Mouse Fab ads/HIgG (gt) (Cat. No. A9917) and an AEC substrate. Antibody dilution 1/4,000.

Properties
Descriptions
Safety Info.
Basic Data

antibody form	ascites fluid
antibody product type	primary antibodies
biological source	mouse
clone	GST-2, monoclonal
conjugate	unconjugated
contains	15 mM sodium azide
isotype	IgG2b
Quality Level	200
shipped in	dry ice
storage temp.	−20°C
target post-translational modification	unmodified
technique(s)	dot blot: suitable
	indirect ELISA: suitable
	western blot: 1:1,000 using purified recombinant GST or lysate of induced bacteria expressing recombinant GST

Application:	Monoclonal Anti-Glutathione-S-Transferase (GST) antibody has been used for use in western blot and ELISA. This product has also been used for dot blot.
Biochem/physiol Actions:	Glutathione S-transferases (GSTs) are a family of enzymes that play an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione.
Disclaimer:	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description:	Monoclonal anti-glutathione-S-transferase (GST) (mouse IgG2b isotype) is derived from the GST-2 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a purified recombinant GST fusion protein.GST belongs to three family of proteins distinguished as cytosolic, mitochondrial and microsomal GST. At present, eight distinct classes of the soluble cytoplasmic mammalian glutathione S-transferases have been identified: α, κ, μ, ω, σ, θ, π and ζ.
General description:	Recombinant target proteins are often expressed as a fusion product with Glutathione-S-Transferase (GST) tags using various expression vector constructs. Thus, antibodies directed against the GST tags of the recombinant constructs can facilitate the purification and study of target proteins. Monoclonal Anti-Glutathione-S-Transferase (GST) antibody reacts with GST from Schistosoma japonicum. Furthermore, the product identifies native as well as denatured-reduced forms of purified GST and GST fusion proteins. The antibody does not detect GST derived from rat, rabbit, porcine and bovine liver or from human placenta.
Immunogen:	recombinant Glutathione-S-Transferase (GST) fusion protein.

Hazard Codes	Xn
Risk Statements	21/22
Safety Statements	36/37-60
RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Storage Temp.	−20°C
UNSPSC	12352203

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