O-Glycosidase from Streptococcus pneumoniae
SIGMA/G1163 - recombinant, expressed in E. coli, buffered aqueous solution
Synonym: Endo-α-N-acetylgalactosaminidase; O-Glycanase
CAS Number: 9032-92-2
EC Number: 232-888-2
MDL Number: MFCD12922510
Product Type: Chemical
Figure 18. Cleavage site and structural requirements for O-Glycosidase. Any modification of the core structure will prevent cleavage.
| concentration | ≥800 units/mL |
| conjugate | (O-linked) |
| form | buffered aqueous solution |
| mol wt | 180 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| shipped in | wet ice |
| storage temp. | 2-8°C |
| Analysis Note: | Screened for presence of: β-galactosidase, α-mannosidase, β-hexosaminidase, α-fucosidase, neuraminidase, and proteases. See Certificate of Analysis for lot specific information. |
| Biochem/physiol Actions: | Releases unsubstituted Ser- and Thr-linked β-Gal-(1→3)-α-GalNAc (Core 1 type O-glycan) from glycoproteins. Substitutions of the disaccharide core with sialic acid, lactosamine (galactose-N-acetyl glucosamine), or fucose will block hydrolysis and prevent the liberation of the oligosaccharide from the protein. Pretreament with glycolytic enzymes to remove substituent saccharides from the O-glycan may be needed prior to cleavage using O-glycosidase.. |
| Other Notes: | One unit will hydrolyze 1 μmole of p-nitrophenyl galacto-N-bioside (β-Gal-(1→3)-α-GalNAc-1→Ο |
| Packaging: | Supplied with 5× Reaction Buffer, 250 mM NaH2PO4 pH 5.0. |
| Physical form: | Solution in 50 mM sodium phosphate, pH 7.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.97 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |