L-Glutamine Synthetase from Escherichia coli
SIGMA/G1270 - lyophilized powder, 400-2,000 units/mg protein
Synonym: L-Glutamate:ammonia ligase (ADP-forming)
CAS Number: 9023-70-5
MDL Number: MFCD00131463
Product Type: Chemical
Unit Definition One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C. Stoichiometry: ATP + L-Glutamate + Ammonia → ADP + Orthophosphate + L-Glutamine
| composition | Protein, ~5% Lowry |
| contains | dithioerythritol as preservative |
| foreign activity | ATPase <0.2% |
| form | lyophilized powder |
| purified by | affinity chromatography |
| Quality Level | 200  |
| solubility | H2O: soluble 0.95-1.05 mg/mL, clear to hazy |
| specific activity | 400-2,000 units/mg protein |
| storage temp. | −20°C |
| UniProt accession no. | P0A9C5  |
| Application: | |
| Application: | L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli. |
| Biochem/physiol Actions: | Degradative enzyme for glutamic acid |
| Biochem/physiol Actions: | L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid. |
| Biochem/physiol Actions: | Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli. |
| General description: | |
| Other Notes: | One unit will convert 1.0 μmole of |
| Physical form: | Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 400-2,000 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 6.3.1.2 ( BRENDA | IUBMB ) |
| UNSPSC | 12352204 |