β-Glucuronidase from Helix pomatia
SIGMA/G1512 - Type H-5, lyophilized powder, ≥400,000 units/g solid
Synonym: β-D-Glucuronide glucuronosohydrolase
CAS Number: 9001-45-0
EC Number: 232-606-8
MDL Number: MFCD00130629
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
LC-MS analysis of codeine-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. The limpet-derived enzyme G8132 performed best with this substrate, with reaction completion within 3–4 hours at 60 °C at 1 unit per mL. Comparative data above was performed at 60 °C at 10 unit per mL.
LC-MS analysis of hydromorphone-glucuronide enzymatic hydrolysis with six different Sigma-produced β-glucuronidase preparations. Molluscan enzymes performed similarly. The reaction was complete within 3–4 hours at 60 °C at 1 unit per mL. The E. coli enzymes exhibited low specificity for this substrate.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G1512-100KU
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G1512-500KU
| form | lyophilized powder |
| Quality Level | 200  |
| secondary activity | ≤40,000 units/g solid sulfatase |
| specific activity | ≥400,000 units/g solid |
| storage temp. | −20°C |
| type | Type H-5 |
| Analysis Note: | Many β-glucuronidases derived from mollusks also contain sulfatase activity. |
| Application: | New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine |
| Application: | β-Glucuronidase from Helix pomatia has been used: • in the enzymatic treatment of urine samples for liquid chromatography-electrospr • in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis • in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS) |
| Application: | β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites. |
| Biochem/physiol Actions: | β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. |
| Biochem/physiol Actions: | Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis |
| General description: | β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity. |
| Other Notes: | A further purification of G0876 by gel permeation chromatography. |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay). |
| Other Notes: | Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C. |
| Packaging: | 100000, 500000 units in poly bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P272 - P280 - P284 - P302 + P352 - P304 + P340 + P312 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥400,000 units/g solid; secondary activity: ≤40,000 units/g solid sulfatase |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |