Glial Cell Line-derived Neurotrophic Factor human
SIGMA/G1777 - recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture, ≥98% (SDS-PAGE)
Synonym: ATF; GDNF
EC Number: 231-509-8
MDL Number: MFCD00285867
Product Type: Chemical
SDS-PAGE Glial Cell Line-derived Neurotrophic Factor Human (Cat. No. G1777) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of GDNF that elicits a 50% attachment factor in cell based bioassay. Cell line: SH-SY5Y.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | endotoxin, tested |
| mol wt | ~30 kDa |
| packaging | pkg of 10 μg |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P39905  |
| Analysis Note: | The biological activity of GDNF is determined by the dose-dependent dopamine uptake by rat ventral mesencephalic cultures. |
| Application: | Glial cell line-derived neurotrophic factor human (GDNF) has been used as a component in the neurobasal medium for neural differentiation. It has also been used for self-renewal, expansion and differentiation of spermatogonial stem cells (SSCs). |
| Biochem/physiol Actions: | Glial cell line-derived neurotrophic factor human (GDNF) acts as a morphogen in kidney development and modulates spermatogonial differentiation. Mutations in this gene may be associated with Hirschsprung′s disease, Tourette syndrome (TS) and attention deficit/ hyperactivity disorder (ADHD). It is used to treat Parkinson′s disease. |
| Biochem/physiol Actions: | Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF is founding member of the GDNF family of ligands, which to date include GDNF, neurturin (NTN), persephin (PSP) and artemin (ART). GDNF is a glycosylated disulfide-linked homodimeric protein of ~15 kDa. Mature rat and human GDNF share ~93% sequence homology, with strong species cross-reactivity. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Using the Parkinson′s disease mouse model, GDNF has been shown to improve conditions such as bradykinesia, rigidity, and postural instability. GDNF promotes survival of various neuronal cells in central and peripheral nervous systems and different stages of development, including motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells. In addition, exogenously applied GDNF has been shown to rescue damaged facial motor neurons in vivo. |
| Biochem/physiol Actions: | Glial Cell Line-Derived Neurotrophic Factor is a member of the cysteine-knot superfamily of growth factors that assume stable dimeric protein structures. GDNF signals through a multicomponent receptor system, composed of a RET and one of the four GFRα (α1-α4) receptors. GDNF specifically promotes dopamine uptake and survival and morphological differentiation of midbrain neurons. Mature human GDNF, a disulfide-linked homodimeric glycoprotein, is predicted to contain two 15 kDa polypeptide chains. |
| General description: | Glial cell line-derived neurotrophic factor (GDNF) human gene is located on human chromosome 5p13.2. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution of 10 mM sodium citrate and 150 mM sodium chloride containing 0.5 mg bovine serum albumin. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H315 - H319 - H335 |
| Precautionary statements | P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xi |
| Risk Statements | 36/37/38 |
| Safety Statements | 26 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |