L-Glutamate Oxidase from Streptomyces sp.

SIGMA/G1924 - recombinant, expressed in E. coli

Synonym: L-Glutamate:oxygen oxidoreductase (deaminating)

CAS Number: 39346-34-4
MDL Number: MFCD00131459
Product Type: Chemical

Unit Definition One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30°C.

Application:	L-Glutamate Oxidase from Streptomyces sp may be used: • as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development • as a component of pencil graphite electrode for biosensing L-glutamate
Biochem/physiol Actions:	L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.
General description:	L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.
General description:	This product has a 6xHistidine tag with a thrombin cleavage site.
Other Notes:	One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: ≥5 units/mg protein
Storage Temp.	−20°C
UNSPSC	12352204