DMT-2′O-Methyl-rG(ib) Phosphoramidite

SIGMA/G211140 - configured for MerMade

Synonym: DMT-2′-O-Me-rG(ib) amidite; N-(2-methyl-1-oxopropyl)-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-guanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]

CAS Number: 150780-67-9
Empirical Formula (Hill Notation): C₄₅H₅₆N₇O₉P
Molecular Weight: 869.94
MDL Number: MFCD00792625
Linear Formula: C₄₅H₅₆N₇O₉P
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G211140-1G	1 g	$111.00 1/EA	Add To Favorites
45-G211140-5G	5 g	$151.00 1/EA	Add To Favorites
45-G211140-10G	10 g	$398.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥99% (³¹P-NMR)
	≥99.0% (reversed phase HPLC)
biological source	non-animal source (no BSE/TSE risk)
color	white to off-white
compatibility	configured for MerMade
form	powder
impurities	≤0.3% mG2 (reversed phase HPLC, Hydrolysate)
	≤0.3% mG3 (reversed phase HPLC, DMT-rG(ib)me)
	≤0.3% water content (Karl Fischer)
	≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR)
	≤0.5% single Impurity (redox titration)
	≤1.0% mG1 (reversed phase HPLC, DMT-rG(ib)me-DMT)
	≤3% residual Solvent content
InChI	1S/C45H56N7O9P/c1-28(2)41(53)49-44-48-40-37(42(54)50-44)47-27-51(40)43-39(57-9)38(61-62(59-25-13-24-46)52(29(3)4)30(5)6)36(60-43)26-58-45(31-14-11-10-12-15-31,32-16-20-34(55-7)21-17-32)33-18-22-35(56-8)23-19-33/h10-12,14-23,27-30,36,38-39,43H,13,25-26H2,1-9H3,(H2,48,49,50,53,54)/t36-,38-,39-,43-,62?/m1/s1
InChI key	IRRDHRZUOZNWDJ-MLLDKZSOSA-N
mol wt	869.94 g/mol
product line	Proligo Reagents
Quality Level	300
SMILES string	CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1n5cnc6C(=O)NC(NC(=O)C(C)C)=Nc56
storage temp.	-10 to -25°C
suitability	conforms to structure for H-NMR
	conforms to structure for LC-MS

General description:

Proligo′s 2′O-Methyl RNA monomers are compatible with fast deprotection schemes that are based on the application of aliphatic amines, such as methylamine. The adenosine and guanosine monomers are protected with the standard benzoyl (bz) and isobutyryl (ib) groups.2′O-Methyl RNA is a nucleic acid analogue that is characterized by the exceptional hybridization properties that it imparts with complimentary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids. The unique combination of properties of 2′O-Methyl RNA had found widespread use in the fields of:• Diagnostic probes
• Aptamer and ribozyme development
• Mixed 2′O-Methyl-RNA/DNA antisense molecule
Key Features: • High yield of crude oligonucleotides
• Compatible with DNA synthesis
• Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides
• Recommended deprotection conditions are 8 hours at 55 °C using concentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C
• Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolyticdegradationDMT-2′O-Methyl-rG(ib) Phosphoramidite is configured for MerMade Synthesizers.

Other Notes:

The synthesis cycle for 2′O-Methyloligoribonucleotides consists of the sameseries of reactions as the cycle that is employed for DNA monomers.However, the rate of coupling for 2′O-Methyl RNA monomers is slowercompared to that of DNA monomers (a coupling time of 6 minutes isrecommended for 2′O-Methyl RNA monomers compared to 90 seconds forDNA monomers). With the exception of the 2′O-Methyl RNA monomers andsupports, RNA synthesis is accomplished with the same reagents as DNAsynthesis. All 2′O-Methyl RNA phosphoramidites from Sigma-Aldrich arediluted with dry acetonitrile.

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
Storage Temp.	-10 to -25°C
UNSPSC	41116105

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