DMT-2′O-Methyl-rG(ib) Phosphoramidite

SIGMA/G211150 - configured for (ÄKTA^® and OligoPilot^®)

Synonym: DMT-2′-O-Me-rG(ib) amidite; N-(2-methyl-1-oxopropyl)-5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-methyl-guanosine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite]

CAS Number: 150780-67-9
Empirical Formula (Hill Notation): C₄₅H₅₆N₇O₉P
Molecular Weight: 869.94
MDL Number: MFCD00792625
Linear Formula: C₄₅H₅₆N₇O₉P
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G211150-5G	5 g	$451.00 1/EA	Add To Favorites
45-G211150-10G	10 g	$902.00 1/EA	Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

λ	conforms (UV/VIS Identity)
assay	≥99% (³¹P-NMR)
	≥99.0% (reversed phase HPLC)
biological source	non-animal source (no BSE/TSE risk)
color	white to off-white
compatibility	configured for (ÄKTA^® and OligoPilot^®)
form	powder
impurities	≤0.1% single unspecified Impurity (reversed phase HPLC)
	≤0.3% mG2 (reversed phase HPLC, Hydrolysate)
	≤0.3% mG3 (reversed phase HPLC, DMT-rG(ib)me)
	≤0.3% water content (Karl Fischer)
	≤0.5% P(III) Impurities 100-169ppm (³¹P-NMR)
	≤1.0% mG1 (reversed phase HPLC, DMT-rG(ib)me-DMT)
	≤3% residual Solvent content
InChI	1S/C45H56N7O9P/c1-28(2)41(53)49-44-48-40-37(42(54)50-44)47-27-51(40)43-39(57-9)38(61-62(59-25-13-24-46)52(29(3)4)30(5)6)36(60-43)26-58-45(31-14-11-10-12-15-31,32-16-20-34(55-7)21-17-32)33-18-22-35(56-8)23-19-33/h10-12,14-23,27-30,36,38-39,43H,13,25-26H2,1-9H3,(H2,48,49,50,53,54)/t36-,38-,39-,43-,62?/m1/s1
InChI key	IRRDHRZUOZNWDJ-MLLDKZSOSA-N
nucleoside profile	base: guanosine base protecting group: isobutyryl 2' protecting group: methyl 5' protecting group: DMT deprotection: standard
product line	Proligo Reagents
Quality Level	300
SMILES string	CO[C@@H]1[C@H](OP(OCCC#N)N(C(C)C)C(C)C)[C@@H](COC(c2ccccc2)(c3ccc(OC)cc3)c4ccc(OC)cc4)O[C@H]1n5cnc6C(=O)NC(NC(=O)C(C)C)=Nc56
storage temp.	-10 to -25°C
suitability	conforms to structure for H-NMR
	conforms to structure for LC-MS
technique(s)	oligo synthesis: suitable

General description:	Proligo′s 2′O-Methyl RNA monomers are compatible with fast deprotectionschemes that are based on the application of aliphatic amines, such asmethylamine. The adenosine and guanosine monomers are protected bythe standard benzoyl (bz) and isobutyryl (ib) groups.2′O-Methyl RNA is a nucleic acid analog that is characterized by theexceptional hybridization properties that it imparts with complimentaryDNA or RNA, as well as increased stability against enzymatic degradationcompared to natural nucleic acids.The unique combination of properties of 2′O-Methyl RNA had foundwidespread use in the fields of:• Diagnostic probes • Aptamer and ribozyme development • Mixed 2′O-Methyl-RNA/DNA antisense molecules Key Features:• High yield of crude oligonucleotides • Compatible with DNA synthesis • Can be employed together with DNA or RNA phosphoramidites in thesame synthesis to produce mixmer oligonucleotides • Recommended deprotection conditions are 8 hours at 55 °C usingconcentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65 °C • Purification and other downstream processing of fully modified 2′OMethyl RNA oligonucleotides are simpler than in the case of RNA, as nospecial precautions are required to provide protection against nucleolyticdegradationDMT-2′O-Methyl-rG(ib) Phosphoramidite is configured for ÄKTA® Synthesizers.
Legal Information:	ÄKTA is a registered trademark of Cytiva
Legal Information:	OligoPilot is a registered trademark of Cytiva

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (³¹P-NMR); ≥99.0% (reversed phase HPLC)
Storage Temp.	-10 to -25°C
UNSPSC	41116105

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