Glucose Oxidase from Aspergillus niger
SIGMA/G2133 - Type VII, lyophilized powder, ≥100,000 units/g solid (without added oxygen)
Synonym: β-D-Glucose:oxygen 1-oxidoreductase; G.Od.; GOx
CAS Number: 9001-37-0
EC Number: 232-601-0
MDL Number: MFCD00063989
Product Type: Chemical
Glucose quantitation: Glucose is oxidized to gluconic acid and hydrogen peroxide by glucose oxidase. Hydrogen peroxide reacts with o-dianisidine in the presence of peroxidase to form a colored product. Oxidized o-dianisidine reacts with sulfuric acid to form a more stable colored product.
| application(s) | diagnostic assay manufacturing |
| composition | Protein, ≥60% |
| does not contain | extender |
| foreign activity | Catalase ≤10 Sigma units/mg protein |
| form | lyophilized powder |
| InChI | 1S/C6H12O6/c7-1-2-3(8)4(9 |
| InChI key | WQZGKKKJIJFFOK-VFUOTHLCSA |
| mol wt | 160 kDa |
| Quality Level | 200  |
| shipped in | wet ice |
| SMILES string | O1[C@H]([C@@H]([C@H]([C@@ |
| specific activity | ≥100,000 units/g solid (without added oxygen) |
| storage temp. | −20°C |
| type | Type VII |
| Analysis Note: | May contain traces of amylase, maltase, glycogenase, invertase, and galactose oxidase. |
| Analysis Note: | Protein determined by biuret. |
| Application: | Glucose oxidase is widely used in the food and pharmaceutical industries as well as a major component of glucose biosensors. |
| Application: | Several publications cite use of the G2133 glucose oxidase in their protocols and in various applications, such as the following: a) Biosensor development: • Diazoresin nanofilm coatings on alginate microspheres: Srivastava, R. et al., Biotechnol. Bioeng., 91(1), 124-131 (2005). • Paper-based glucose biosensor: Lankelma, J. et al., Anal. Chem., 84(9), 417-4152 (2012) • Microfluidic device with glucose oxidase immobilized on hydrogel for glucose analysis of blood: He, R.-Y. et al., RSC Adv., 9, 32367-32374 (2019). b) Single-molecule FRET study of human RAD51 filament formation: Subramanyam, S. et al., Methods Enzymol., 600, 201-232 (2018). c) Enzymatic fuel-cells with chitosan-based membranes: Bahar, T., and Yazici, M.S., Electroanalysis, 32(6), 1304-1314 (2020). |
| Biochem/physiol Actions: | Glucose oxidase catalyses the oxidation of β-d-glucose to d-glucono-β-lactone and hydrogen peroxide, with molecular oxygen as an electron acceptor. |
| General description: | Molecular Weight: 160 kDa (gel filtration) pI: 4.2 Extinction coefficient: E1% = 16.7 (280 nm) Glucose oxidase from Aspergillus niger is a dimer consisting of 2 equal subunits with a molecular mass of 80 kDa each. Each subunit contains one flavin adenine dinulceotide moiety and one iron. The enzyme is a glycoprotein containing ~16% neutral sugar and 2% amino sugars. The enzyme also contains 3 cysteine residues and 8 potential sites for N-linked glycosylation. Glucose oxidase is capable of oxidizing The pH optimum for glucose oxidase is 5.5, while it has a broad activity range of pH 4-7. Glucose oxidase is specific for β- Glucose oxidase does not require any activators, but it is inhibited by Ag+, Hg2+, Cu2+, phenylmercuric acetate, and p-chloromercuribenzoate. It is not inhibited by the nonmetallic SH reagents: N-ethylmaleimide, iodoacetate, and iodoacetamide. Glucose oxidase can be utilized in the enzymatic determination of |
| Other Notes: | One unit will oxidize 1.0 μmole of β- |
| Physical form: | Lyophilized powder containing phosphate buffer salts and sodium chloride |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| Hazard Codes | Xn |
| Risk Statements | 42 |
| Safety Statements | 22-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| activity | specific activity: ≥100,000 units/g solid (without added oxygen) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 1.1.3.4 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |