L-Glutamine solution

SIGMA/G2150 - 200 mM, Hybri-Max^™, sterile-filtered, suitable for hybridoma

Synonym: L-Glutamine for cell culture

CAS Number: 56-85-9
MDL Number: MFCD00008044
Product Type: Chemical

Catalog Number	PKG Qty.	Price	Quantity


45-G2150-20ML	20 mL	$35.90 1/EA		Add To Favorites

Properties
Descriptions
Safety Info.
Basic Data

assay	≥99% (TLC)
color	clear colorless
form	solution
grade	Hybri-Max^™
impurities	endotoxin, tested
InChI	1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1
InChI key	ZDXPYRJPNDTMRX-VKHMYHEASA-N
Quality Level	300
shipped in	dry ice
SMILES string	N[C@@H](CCC(N)=O)C(O)=O
sterility	sterile-filtered
storage temp.	−20°C
technique(s)	cell culture \| hybridoma: suitable

Application:	The L-Glutamine solution has been used as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture human leukemia monocytic cell line for intracellular assay and splenocytes for in vitro activation of CD4⁺ T cells. It has also been used as a supplement to the hybridoma medium used for primary screening for antigen binding and hybridoma cloning.
General description:	L-glutamine is an essential amino acid that is a crucial component of culture media that serves as a major energy source for cells in culture. L-glutamine is very stable as a dry powder and as a frozen solution. In liquid media or stock solutions, however, L-glutamine degrades relatively rapidly. Optimal cell performance usually requires supplementation of the media with L-glutamine prior to use.
Legal Information:	Hybri-Max is a trademark of Sigma-Aldrich Co. LLC
Preparation Note:	Prepared in cell culture grade water.

RIDADR	NONH for all modes of transport
WGK Germany	nwg
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

Purity	≥99% (TLC)
Storage Temp.	−20°C
UNSPSC	12352209

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