β-Glucuronidase from limpets (Patella vulgata)
SIGMA/G2174 - aqueous solution, ≥85,000 units/mL
Synonym: β-D-Glucuronide glucuronosohydrolase
CAS Number: 9001-45-0
MDL Number: MFCD00130629
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
| biological source | limpet (Patella vulgata) |
| form | aqueous solution |
| Quality Level | 200  |
| secondary activity | ≤7,500 units/mL sulfatase |
| specific activity | ≥85,000 units/mL |
| storage temp. | 2-8°C |
| Application: | New Technical Article Comparing Performance of Different Enzymes Learn more about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine |
| Application: | The enzyme from patella vulgata is reported to be more effective in hydrolyzing opioid-glucuronides than the Helix pomatia, bovine liver and E. coli enzymes |
| Features and Benefits: | Save time with no reconstitution in this convenient liquid format. |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 3.8 (30 min assay). |
| Other Notes: | Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C. |
| Physical form: | Aqueous solution in 0.9% NaCl with 0.02% sodium azide as preservative. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 23-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥85,000 units/mL; secondary activity: ≤7,500 units/mL sulfatase |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 3.2.1.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |