Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

SIGMA/G2267 - lyophilized powder, ≥75 units/mg protein

Synonym: D-Glyceraldehyde 3-phosphate:NAD⁺ oxidoreductase (phosphorylating); GAPDH

CAS Number: 9001-50-7
EC Number: 232-609-4
MDL Number: MFCD00131203
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G2267-500UN	500 units	$76.90 1/EA	Add To Favorites
45-G2267-1KU	1000 units	$114.00 1/EA	Add To Favorites
45-G2267-5KU	5000 units	$391.00 1/EA	Add To Favorites
45-G2267-10KU	10000 units	$688.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

biological source	rabbit muscle
composition	Protein, ≥80% biuret
foreign activity	3-Phosphoglyceric phosphokinase ≤0.05%
	lactic dehydrogenase, myokinase, and pyruvate kinase ≤0.01%
	triosephosphate isomerase ≤0.1%
form	lyophilized powder
Quality Level	200
shipped in	wet ice
specific activity	≥75 units/mg protein
storage temp.	−20°C

Application:	Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle has been used: • for measurements of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) activity assay • to generate a linear standard curve to analyse GAPDH activity in the experimental samples collected from mice • in anti-aggregation assays • in purified GAPDH studies
Biochem/physiol Actions:	Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate into D-glycerate-1,3-bisphosphate as part of the glycolysis pathway.
Biochem/physiol Actions:	Glyceraldehyde-3-phosphate dehydrogenase catalyzes the conversion of glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate as part of glycolysis. It has also been shown to have roles in initiation of apoptosis, transcription activation and the shuttling of ER to Golgi vesicles<<<18,New>>>.
General description:	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a multifunctional protein. It is basically called as a glycolytic enzyme.
Other Notes:	One unit will reduce 1.0 μmole of 3-phosphoglycerate to D-glyceraldehyde 3-phosphate per min in a coupled system with 3-phosphoglyceric phosphokinase at pH 7.6 at 25 °C.
Packaging:	1000, 5000, 10000 units in poly bottle
Packaging:	500 units in glass bottle
Physical form:	Lyophilized powder containing citrate buffer salts

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: ≥75 units/mg protein
Storage Temp.	−20°C
Enzyme Commission (EC) Number	1.2.1.12 ( BRENDA \| IUBMB )
UNSPSC	12352204

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