Monoclonal Anti-β-COP antibody produced in mouse
SIGMA/G2279 - clone M3A5, ascites fluid
Synonym: Anti-BARMACS; Anti-COPB
MDL Number: MFCD00164606
Product Type: Chemical
Immunofluorescence Chinese hamster ovary (CHO) cells were fixed and permeabilized with methanol followed by acetone. Fixed cells were stained with 1:40 Anti-β-COP antibody, Mouse monoclonal, Clone: M3A5 (Cat. No. G2279) followed by 1:40 Anti-Mouse IgG (Fab specific)-FITC antibody produced in Goat (Cat. No. F5262).
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | M3A5, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | monkey, human, chicken, goose, rabbit, canine, bovine, kangaroo rat, rat, hamster |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): suitable | |
| indirect immunofluorescence: 1:20 using cultured Chinese hamster ovary (CHO) cells | |
| UniProt accession no. | P53618  |
| Application: | Monoclonal Anti-β-COP antibody produced in mouse has been used: • for the localization of β-COP using immunoprecipitation • in immunocytochemistry • in immunoblotting • with other antibodies to Golgi proteins to study the role and relationships of this protein in the cell |
| Biochem/physiol Actions: | COPs (coatomer proteins) are transiently attached to the vesicles involved in transport within the Golgi complex and possibly between the rough ER and Golgi complex. |
| Biochem/physiol Actions: | The antibody recognizes an epitope shared by β-COP (110 kDa) found in most tissue culture lines, and by a doublet of brain microtubule-associated protein (MAP2, 270-300 kDa). The antibody stains a reticular structure in the perinuclear area of non-neuronal cells (the periphery of Golgi complex and a population of coatomers scattered throughout the cytoplasm) in tissues from different species and cell processes, as well as cell bodies in chicken brain neuronal cells. It has been used for studies on the effects of various agents that influence energy status, disrupt the Golgi complex, or alter the activity of G-proteins or small GTP-binding proteins on the cellular localization of β-COP. The antibody may be used for the immunoaffinity purification of the protein. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Monoclonal Anti- β-COP (mouse IgG1 isotype) is derived from the M3A5 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. COPs (coatomer proteins) have a molar mass of 110 kDa and its primary structure is homologous to the β-adaptin component of clathrin-coated vesicles. |
| General description: | The coatomer (approx. 550kDa) consists of proteins designated α-, β-, γ-, and δ-COP, together with substoichiometric amounts of several other proteins. |
| Immunogen: | microtubule-associated protein from goose brain. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |