Glutamic-Oxalacetic Transaminase from porcine heart

SIGMA/G2751 - Type I, ammonium sulfate suspension, 200-500 units/mg protein

Synonym: L-Aspartate:2-oxoglutarate aminotransferase; Aspartate Aminotransferase; GOT

CAS Number: 9000-97-9
EC Number: 232-571-9
MDL Number: MFCD00131191
Product Type: Chemical

Catalog Number	PKG Qty.	Price


45-G2751-1KU	1000 units	$188.00 1/EA	Add To Favorites
45-G2751-2KU	2000 units	$351.00 1/EA	Add To Favorites
45-G2751-10KU	10000 units	$1390.00 1/EA	Add To Favorites

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Properties
Descriptions
Safety Info.
Basic Data

foreign activity	glutamic-pyruvic transaminase ≤0.03%
	lactic dehydrogenase ≤0.01%
	malic dehydrogenase ≤0.01%
form	ammonium sulfate suspension
Quality Level	200
shipped in	wet ice
specific activity	200-500 units/mg protein
storage temp.	2-8°C
type	Type I

Analysis Note:	Protein determined by biuret.
Application:	Glutamic-Oxalacetic Transaminase from porcine heart has been used: • in an enzyme-coupled assay for evaluating L-asparaginase enzyme activity • in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples • as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system
Application:	Glutamic-OxalaceticTransaminase from porcine heart is suitable for the preparation ofphenylalanine amino acid. The immobilized form of this enzyme maybe used incomparison studies between soluble and immobilized forms in terms of reactionefficiency and stability.
Biochem/physiol Actions:	Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L-aspartate and α-ketoglutarate with oxalacetate and L-glutamate. This reversible transaminase reaction is dependent on pyridoxal 5′-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L-glutamate. Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.
General description:	Research area: Cellsignaling. Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle. It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.
Other Notes:	One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.5 at 37 °C, in the presence of L-aspartic acid. One unit is equivalent to ~2,000O.D. (Karmen) units at 25 °C.
Packaging:	10000 units in serum bottle
Physical form:	Suspension in 3.0 M (NH₄)₂SO₄ containing 0.05 M maleate and 2.5 mM α-ketoglutarate, pH 6.0

RIDADR	NONH for all modes of transport
WGK Germany	WGK 3
Flash Point(F)	Not applicable
Flash Point(C)	Not applicable

activity	specific activity: 200-500 units/mg protein
Storage Temp.	2-8°C
Enzyme Commission (EC) Number	2.6.1.1 ( BRENDA \| IUBMB )
UNSPSC	12352204

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