Anti-Glutamine Synthetase antibody produced in rabbit
SIGMA/G2781 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Glutamine Synthetase Antibody; Glutamine Synthetase Antibody - Anti-Glutamine Synthetase antibody produced in rabbit
MDL Number: MFCD01322097
Product Type: Chemical
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat brain sections with 1:10,000 Rabbit Anti-Glutamine Synthetase (Cat. No. G2781) using biotin/ExtrAvidin®-Peroxidase.
Immunohistochemistry Formalin-fixed, paraffin-embedded tissue section of rat brain stained with 1:10,000 Anti-Glutamine Synthetase antibody produced in Rabbit (Cat. No. G2781) followed by Anti-Rabbit IgG-Biotin antibody produced in Goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Immunoblotting Rat brain cytosolic S1 fraction was separated on SDS-PAGE and probed with IGG FRACTION ANTI GLUTAMINE SYNTHETASE (RB) (Cat. No. G2781). The antibody was developed with Alk Phos APA Rabbit IgG (gt) (Cat. No. A9919) and a NBT/BCIP substrate. Antibody dilution 1/20,000.
Immunoblotting Whole extract of rat brain was separated on SDS-PAGE and probed with Rabbit Anti-Glutamine Synthetase (Cat. No. G2781). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:5,000 2. Antibody dilution 1:10,000 3. Antibody dilution 1:20,000
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 45 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200  |
| species reactivity | rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10,000 using rat brain sections |
| microarray: suitable | |
| western blot: 1:10,000 using rat brain cytosolic fraction | |
| UniProt accession no. | P09606  |
| Application: | Immunoblotting: a minimum working antibody dilution of 1:10,000 is determined using a rat brain cytosolic fraction extract. Immunohistochemistry: a minimum working antibody dilution of 1:10,000 is determined using formalin-fixed, paraffin-embedded sections of rat brain. |
| Application: | Anti-Glutamine Synthetase antibody has been used for the detection and localization of GS by immunoblotting and immunohistochemistry. |
| Biochem/physiol Actions: | Anti-Glutamine synthetase recognizes rat glutamine synthetase (GS) (45 kDa). Staining of GS in immunoblotting is specifically inhibited with the GS immunizing peptide (amino acids 357-373 with Nterminally added lysine). |
| Biochem/physiol Actions: | Glutamine synthetase (GS) catalyzes the amidation of glutamate to form glutamine. In the brain, GS is considered as a key enzyme, participating in recycling of the excitatory neurotransmitter glutamate released from neurons and termination of the neurotransmitter signal, as well as in ammonia detoxification. GS activity is a useful marker for astrocytes and an important differentiation feature in the retina. Upregulation of GS is a hallmark of reactive astrocytosis and GS activity level increases following spinal cord injury. The GS expression in astrocytes is diminished in brains affected by Alzheimer′s disease, particularly in the vicinity of senile plaques. GS is upregulated in a subset of human hepatocellular carcinomas, suggesting that its expression may be related to tumor recurrence, since tumors expressing GS are independent of the extracellular glutamine supply. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Anti-Glutamine Synthetase is produced in rabbit using a synthetic peptide corresponding to the C-terminus of mouse glutamine synthetase (GS) conjugated to keyhole limpet hemocyanin (KLH) as immunogen. This sequence is identical in human, bovine, rat, hamster and pig GS, and highly conserved in chicken GS. GS is found in mammals as an octamer of identical 45 kDa subunits. It is localized in glial cells of various species. It is present in the brain primarily in astrocytes and in the retina in Muller glial cells that support neurons using glutamate as a neurotransmitter. GS is found in several brain regions including hippocampus, cerebral cortex, neostriatum and cerebellar granular layer, corresponding to sites with high glutamate receptor density. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region of mouse glutamine synthetase (GS) (amino acids 357-373. This sequence is identical in human, bovine, rat, hamster, and pig GS and highly conserved chicken Gs (single amino acid substitution). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |