Monoclonal Anti-Goat/Sheep IgG antibody produced in mouse
SIGMA/G2904 - clone GT-34, ascites fluid
Synonym: Monoclonal Anti-Goat/Sheep IgG
Product Type: Chemical
ELISA Monoclonal Anti-Goat/Sheep IgG antibody produced in Mouse, Clone: GT-34 (Cat. No. G2904) followed by 1:1,000 Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in Goat (Cat. No. A9917) was probed to detect Goat/Sheep IgG by indirect ELISA. Reactivity is observed with Goat IgG and Sheep IgG but not with Bovine IgG and Human IgG.
| antibody form | ascites fluid |
| antibody product type | secondary antibodies |
| biological source | mouse |
| clone | GT-34, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgG1 |
| Quality Level | 200  |
| shipped in | dry ice |
| should not react with | rat, canine, mouse, rabbit, guinea pig, chicken, horse, pig, feline, human |
| species reactivity | sheep, goat, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect ELISA: 1:10,000-1:20,000 |
| Application: | Monoclonal Anti-Goat/Sheep IgG antibody produced in mouse has been used in: • enzyme linked immunosorbent assay (ELISA) • immunoblotting • immunoprecipitation • immunoelectrophoresis |
| Biochem/physiol Actions: | IgG stimulates the classical pathway of the complement system. It neutralizes virus particles and toxins. IgG plays a crucial role in antibody-dependent cell-mediated cytotoxicity (ADCC). IgG might be associated with allergy. It has the longest serum half life. |
| Biochem/physiol Actions: | Monoclonal Anti-Goat/Sheep IgG recognizes an epitope located on the heavy chain of goat IgG1 and IgG2. The antibody shows strong cross-reactivity with sheep IgG and weak cross-reactivity with bovine IgG. No cross-reactivity is observed with IgG derived from human, guinea pig, rat, horse, dog, chicken, pig, mouse, rabbit, or cat. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | IgG is the main type of antibody in human serum, secreted by the B cells. It is found in blood. IgG consists of four polypeptide chains, made of two identical 50 kDa γ heavy (H) chains and two identical 25 kDa κ or λ light (L) chains, which are linked by inter-chain disulfide bonds. IgG contains four isotypes IgG1, IgG2, IgG3 and IgG4. |
| General description: | The antibody reacts with an epitope located on the heavy chain of goat IgG1 and IgG2. |
| Physical form: | The product is provided as ascites fluid with 0.1% sodium azide as preservative. |
| Preparation Note: | For continuous use, store at 2–8 °C for up to one month. For extended storage, solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify by centrifugation before use. |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |