GenElute™ Plant Genomic DNA Miniprep Kit
SIGMA/G2N350 - sufficient for 350 purifications
Synonym: Plant Genomic DNA Miniprep; Gen Elute
Product Type: Chemical
Genomic DNA from various plant species isolated with GenElute™ Plant Genomic DNA Miniprep Kit.
Purified genomic DNA (0.4 μg/lane) was analyzed on a 0.8% agarose gel. Marker is lambda Hind III digest.
Purified genomic DNA (0.4 μg/lane) was analyzed on a 0.8% agarose gel. Marker is lambda Hind III digest.
Genomic DNA isolated from 100 mg fresh tomato leaves using various kits.
Purified genomic DNA (0.4 μg/lane) was analyzed on a 0.8% agarose gel. Sigma, membrane-based; Supplier A, resin-based; Supplier M, solution based; Supplier Q, membrane-based. Marker is lambda Hind III digest.
Note: RNA contamination is present in DNA isolated using the kits from both Supplier A and M.
Purified genomic DNA (0.4 μg/lane) was analyzed on a 0.8% agarose gel. Sigma, membrane-based; Supplier A, resin-based; Supplier M, solution based; Supplier Q, membrane-based. Marker is lambda Hind III digest.
Note: RNA contamination is present in DNA isolated using the kits from both Supplier A and M.
PCR amplification of a 500 bp product isolated from genomic DNA.
Genomic DNA from soybean leaves was purified using the GenElute™ Plant Genomic DNA Miniprep Kit. A 5 μL aliquot of eluate was used as template in a 20 μL total PCR reaction for 30 cycles. A 5 μL aliquot of each PCR reaction was resolved on a 2% precast agarose gel (Cat. No. P5722). The PCR marker (M) used (Cat. No. P9577) ranged from 50 bp to 2 kb.
Genomic DNA from soybean leaves was purified using the GenElute™ Plant Genomic DNA Miniprep Kit. A 5 μL aliquot of eluate was used as template in a 20 μL total PCR reaction for 30 cycles. A 5 μL aliquot of each PCR reaction was resolved on a 2% precast agarose gel (Cat. No. P5722). The PCR marker (M) used (Cat. No. P9577) ranged from 50 bp to 2 kb.
Comparison of preparation time.
The prep time required using the GenElute™ Plant Genomic DNA Miniprep Kit, compared to kits from three other suppliers.
The prep time required using the GenElute™ Plant Genomic DNA Miniprep Kit, compared to kits from three other suppliers.
12 Principles of Green Chemistry: Principle 12—Inherently Safer Chemistry for Accident Prevention. This product utilizes safer chemistry to minimize the potential for chemical accidents, including releases, explosions and fires.
| greener alternative category |  , Aligned |
| greener alternative product characteristics | Inherently Safer Chemistry for Accident Prevention Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| storage temp. | 15-25°C |
| sustainability | Greener Alternative Product |
| technique(s) | DNA purification: suitable |
| usage | sufficient for 350 purifications |
| Application: | The purified genomic DNA is ready for immediate use in sensitive downstream applications such as: • PCR • restriction endonuclease digestion • cloning • Southern blots |
| Biochem/physiol Actions: | Several micrograms of DNA can be obtained from up to 100 mg of fresh tissue or 10 mg of freeze-dried material in less than an hour. Plant tissue is disrupted by grinding in liquid nitrogen and the DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified DNA is greater than 20 kb in length. |
| Features and Benefits: | • Starting material: Up to 100 mg of plant tissue • Expected yield: Up to 20 μg • Elution volume: 100 - 200 μl • Time required: < 40 min • RNase treatment required: No |
| General description: | Click here to view the kit protocol |
| General description: | The GenElute Plant Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure DNA from a variety of plant species. The GenElute kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform. |
| General description: | The GenElute Plant Genomic DNA Purification Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of plant species. This kit combines the advantages of a silica-based system with a microspin format and eliminates the need for expensive resins, RNase treatment, and hazardous organic compounds such as phenol and chloroform. Plant tissue is disrupted by grinding in liquid nitrogen, and DNA is released with detergent and chaotrope. Proteins, polysaccharides, and cell debris are eliminated with a 10 minute precipitation procedure followed by centrifugation through a filtration column, included in the kit. The genomic DNA is purified further by a silica bind-wash-elute procedure in microcentrifuge spin columns. The purified genomic DNA is ready for immediate use in downstream applications such as PCR, restriction digestion, cloning and Southern blots. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | For additional information, please see www.sigma-aldrich.com/gen. |
| Symbol |   GHS05,GHS07 |
| Signal word | Danger |
| Hazard statements | H290 - H302 + H332 - H314 - H412 |
| Precautionary statements | P273 - P280 - P301 + P312 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 |
| RIDADR | UN 3316 9 |
| Supplemental Hazard Statements | EUH032 |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |
| Components | Column Preparation Solution |