β-Galactosidase from Escherichia coli
SIGMA/G3153 - lyophilized powder, ≥500 units/mg protein
Synonym: β-D-Galactoside galactohydrolase; Lactase
CAS Number: 9031-11-2
EC Number: 232-864-1
MDL Number: MFCD00155434
Product Type: Chemical
This image is provided for informative purposes only and does not represent an actual product label. It should not be used as a substitute for official product labeling.
| form | lyophilized powder |
| mol wt | 465 kDa |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| specific activity | ≥500 units/mg protein |
| storage temp. | −20°C |
| Application: | β-Galactosidase from Escherichia coli has been used to produce biocatalytic Ca-alginate beads on a B-390 encapsulator (BUCHI). It has been used to prepare the source droplet solution and also as the cargo for Pep-1 (KETWWETWWTEWSQPKKKRKV-cy |
| Application: | β-Galactosidase may be used for derivatization, such as an enzyme label for IgG, without prior dialysis or gel filtration. |
| Biochem/physiol Actions: | β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop. |
| Biochem/physiol Actions: | β-galactosidase is considered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure and target gene expression. |
| General description: | Tetramer molecular weight 465 kDa (subunits 116.3 kDa each) |
| General description: | β-galactosidase is a 465 kDa tetrameric protein. It is coded by the LacZ gene. |
| Other Notes: | One unit will hydrolyze 1.0 μmole of o-nitrophenyl β- |
| Packaging: | Package size based on protein content |
| Physical form: | Stabilized with phosphate buffer and sucrose |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥500 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.23 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |