Anti-GADD45A (12-25) antibody produced in rabbit
SIGMA/G3548 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-DDIT1; Anti-GADD45; Anti-Growth arrest and DNA-damage-inducible protein GADD45 α
Product Type: Chemical
Immunofluorescence Anti-GADD45A (12-25), Cat. No. G3548: Immunofluorescence of HUVEC cells using Anti-GADD45A (12-25), G3548 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
Immunoblotting Lysate of HEK-293T cells overexpressing human GADD45A was separated on SDS-PAGE, blotted with Anti-GADD45A (12-25) (Cat. No. G3548) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:500 2. Antibody dilution 1:500 + GADD45A immunizing peptide (human, 12-25) 3. Antibody dilution 1:1,000
Immunofluorescence Graph Anti-GADD45A (12-25), Cat. No. G3548: Intensity analysis of Anti-GADD45A (12-25) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different Antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~18 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1:500-1:2,000 |
| UniProt accession no. | P24522  |
| Application: | Anti-GADD45A (12-25) antibody produced in rabbit has been used in immunocytochemistry. |
| Application: | Anti-GADD45A antibody produced in rabbit is suitable for western blotting at a working dilution of 1:500-1:2000. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if . |
| Biochem/physiol Actions: | GADD45A (growth arrest and DNA-damage-inducible, alpha) gene encodes a protein that belongs to a group of genes that are expressed under stressful growth arrest conditions and upon treatment with DNA-damaging agents such as methylmethane sulfonate and UV radiation. The protein mediates activation of the p38/JNK pathway via MTK1/MEKK4 kinase upon environmental stress. It inhibits entry of cells into S phase by activating DNA excision repair in vitro. It plays a crucial role in cell cycle checkpoints, DNA repair, and genome integrity maintenance. |
| Biochem/physiol Actions: | Growth arrest and DNA damage inducible α (GADD45A) miRNAs are differentially expressed in osteosarcoma. It interacts with proliferating cell nuclear antigen (PCNA), aurora A kinase and histones. GADD45A improves the cytotoxic effect of the drug temozolomide (TMZ) in glioblastoma multiforme (GBM). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Growth arrest and DNA damage inducible α It is expressed at low levels in testis, but, at moderate level in liver, brain, heart and spleen. GADD45A shows high expression in kidney and skeletal muscle. |
| Immunogen: | synthetic peptide corresponding to amino acids 12-25 of human GADD45A |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |