Guanosine 5′-triphosphate sodium salt solution
SIGMA/G3776 - HPLC purified, aqueous solution for RNA polymerase transcription
Synonym: GTP
CAS Number: 36051-31-7
MDL Number: MFCD00077781
Product Type: Chemical
Representative packaging
| assay | ≥95% (HPLC) |
| biological source | bacterial (Corynebacterium) |
| Porcine brain | |
| yeast | |
| color | colorless |
| concentration | 100 mM in H2O (adjusted to pH 7) |
| foreign activity | DNase, RNase and Nickase, none detected |
| form | liquid |
| InChI | 1S/C10H16N5O14P3.3Na/c11- |
| InChI key | KZRMTEVIDYXWQW-CYCLDIHTSA |
| Quality Level | 300  |
| shipped in | dry ice |
| SMILES string | [Na+].[Na+].[Na+].NC1=Nc2 |
| storage temp. | −20°C |
| Application: | Guanosine 5′-triphosphate sodium salt solution has been used: • for reconstituting transducin during purification of transducin. • as a compnent of the 3.3X loading buffer to assemble 32P-miR-31—3xFLAG-AGO2 complexes • as a component of the nucleotide substrate mix to evaluate the hydrolytic activity of MutT homolog 1 (MTH1) on methylated nucleoside triphosphates • in DNA-dependent RNA polymerase transcription |
| Biochem/physiol Actions: | Guanosine 5′-triphosphate (GTP) forms nearly 25% of the total nucleotide triphosphate pool and is a nucleotide anion. It participates in several biological functions such as cell signaling by activation of GTP-binding proteins, RNA and protein synthesis, and production of cyclic guanosine monophosphate (cGMP), a secondary messenger. GTP also participates in the production of stringent alarmone. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥95% (HPLC) |
| Storage Temp. | −20°C |
| UNSPSC | 41106305 |