Anti-Glucocerebrosidase antibody produced in rabbit
SIGMA/G4046 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Glucocerebrosidase antibody produced in Rabbit (Cat. No. G4046) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Glucocerebrosidase (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Immunoblotting Whole cell lysates were separated on SDS-PAGE and blotted with Anti-Glucocerebrosidase (Cat. No. G4046). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:1,000 Lanes 1,3. HEK293 cells co-transfected with human glucocerebrosidase 2. HEK293 cells (untransfected) 3. Inhibition with glucocerebrosidase immunizing peptide (human, 83-100) (2 μg/mL)
Immunoblotting Cell extracts were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-Glucocerebrosidase (Cat. No. G4046) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. SK-N-SH; 2. SH-SY-5Y; 3. SH-SY-5Y. Antibody + immunizing glucocerebrosidase peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~60 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using HEK-293T cell lysate expressing human glucocerebrosidase (GBA) or SH-SY-5Y cell lysate |
| UniProt accession no. | P04062  |
| Application: | Anti-Glucocerebrosidase antibody produced in rabbit has been used in western blotting and immunocytochemistry. |
| Application: | Anti-Glucocerebrosidase antibody produced in rabbit is suitable for immunoblotting at a working concentration of 1-2μg/mL using HEK-293T cell lysate expressing human glucocerebrosidase (GBA). |
| Biochem/physiol Actions: | GBA (glucosidase, beta, acid) gene encodes a protein that cleaves the β-glucosidic linkage of glycosylceramide (GlcCer), an intermediate in glycolipid metabolism. Mutations in the human GBA gene cause a reduction in the GBA activity and accumulation of GlcCer in lysosomes of cells of the reticuloendothelial system. This causes Gaucher disease (GD), an inherited lysosomal storage disorder, characterised by severe loss of neurons in the central nervous system, fetal onset, hydrops fetalis, in utero fetal death and neonatal distress. It leads to changes in neuronal functionality including increased levels of tubular endoplasmic reticulum (ER) elements, a large increase in Ca2+ release from the ER in response to glutamate, and an increased sensitivity to glutamate-induced neurotoxicity. Mutations in the human GBA gene may contribute to the development of common age-related dementia known as dementia with Lewy bodies or DLB and may cause early-onset of Parkinson disease. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GBA1 (β-glucocerebrosidase) gene is mapped to human chromosome 1q21. It is a lysosomal enzyme and is widely expressed. The protein has domain I with three stranded anti?parallel βsheets, domain II with two β sheets making an immunoglobulin like domain and domain III with eight stranded β/α triosephosphate isomerase (TIM) barrel. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |