Anti-Glucocerebrosidase (C-terminal) antibody produced in rabbit
SIGMA/G4171 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-
Product Type: Chemical
Enhanced Validation-By Recombinant Expression Antibody recognition: Anti-Glucocerebrosidase (C-terminal) antibody produced in Rabbit (Cat. No. G4171) is confirmed by western blot in the cell sample that has been altered to overexpress the target protein-HEK-293T over expressing Glucocerebrosidase (Lane 1) while no staining is detected in the wild type sample-HEK-293T (Lane 2). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate. Antibody dilution: 1:1,000.
Immunoblotting Whole cell lysates were separated on SDS-PAGE and blotted with Anti-Glucocerebrosidase (C-terminal) (Cat. No. G4171). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and chemiluminescent substrate. Antibody dilution 1:1,000 Lanes 1,3. HEK293 cells co-transfected with human glucocerebrosidase 2. HEK293 cells (untransfected) 3. Inhibition with glucocerebrosidase immunizing peptide (human, 517-536) (2 μg/mL)
Immunoblotting Cell and tissue extracts were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-Glucocerebrosidase (C-terminal) (Cat. No. G4171) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. Rat brain (S1); 2. SK-N-SH; 3. SH-SY-5Y; 4. SH-SY-5Y. Antibody + immunizing glucocerebrosidase peptide
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| enhanced validation | recombinant expression Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen ~60 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 1-2 μg/mL using HEK293-T cells lysate expressing human glucocerebrosidase (GBA) |
| UniProt accession no. | P04062  |
| Application: | Anti-glucocerebrosidase (C-terminal) antibody can be used in western blotting and immunoblotting. |
| Application: | Anti-Glucocerebrosidase (C-terminal) antibody produced in rabbit has been used in • immunohistochemistry • immunoblot analysis |
| Biochem/physiol Actions: | Glucocerebrosidase (GBA) activity is reduced in human with mutations in GBA gene and causes accumulation of glucosylceramide (GlcCer). Fibroblasts from patients with defined GBA mutations show either retarded or blocked transport of GBA in the endoplasmic reticulum. Mutations in the human GBA gene may contribute to the development of common age-related dementia known as dementia with Lewy bodies. Several studies indicate that mutations in the human GBA gene are associated with early-onset Parkinson disease. |
| Biochem/physiol Actions: | Glucocerebrosidase is an enzyme having glucosylceramidase activity. Defect in lysosomal hydrolase glucocerebrosidase results in Gaucher disease. Mutation in GBA will retard or block the transport of GBA to endoplasmic reticulum. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Glucocerebrosidase is an enzyme having glucosylceramidase activity. Defect in lysosomal hydrolase glucocerebrosidase results in Gaucher disease. Mutation in GBA will retard or block the transport of GBA to endoplasmic reticulum. Anti-glucocerebrosidase (C-terminal) antibody can be used to incubate the SDS-PAGE gel. Anti-Glucocerebrosidase antibody reacts specifically with human GBA. |
| Immunogen: | synthetic peptide corresponding to amino acids 517-536 of human glucocerebrosidase (GBA), conjugated to KLH. This sequence is identical in rat GBA and highly conserved in mouse GBA (single amino acid substitution). |
| Other Notes: | 2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |