Glyoxalase I from Saccharomyces cerevisiae
SIGMA/G4252 - Grade IV, buffered aqueous glycerol solution, ≥400 units/mg protein
Synonym: S-
CAS Number: 9033-12-9
MDL Number: MFCD00131225
Product Type: Chemical
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| foreign activity | glyoxalase II ≤1% |
| form | buffered aqueous glycerol solution |
| Quality Level | 200  |
| specific activity | ≥400 units/mg protein |
| storage temp. | 2-8°C |
| type | Grade IV |
| Analysis Note: | Protein determined by biuret. |
| Application: | Glyoxalase I from Saccharomyces cerevisiae has been used as a standard in Glyoxalase I assay for C. dilatata females and brooded encapsulated embryos. It has also been used as a standard for calibration curve generation for quantifying glyoxalase I from brain tissues and cerebral microvessels. |
| Biochem/physiol Actions: | Glyoxalase I is universally expressed and involved in the protection against cellular damage due to cytotoxic metabolites such as advanced glycation end products (AGEs). It is an integral component of the detoxification system, catalyzing the conversion of reactive, acyclic a-oxoaldehydes into the corresponding a-hydroxyacids in a glutathione-dependent manner. |
| General description: | Glyoxalase detoxification system consists of glyoxalase (GLO)-I and GLO-II. GLO-I is a cytosolic, 42 kDa, dimeric Zn2+ metalloenzyme. |
| Other Notes: | One unit will form 1.0 μmole of S-lactoylglutathione from methylglyoxal and reduced glutathione per min at pH 6.6 at 25 °C. |
| Packaging: | 500, 1000, 2500 units in glass bottle |
| Physical form: | Solution in 50% glycerol, 0.4 M (NH4)2SO4 and 0.002 M KH2PO4 pH 6.5 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥400 units/mg protein |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 4.4.1.5 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |