Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast
SIGMA/G4256 - Type IV, lyophilized powder, 20-60 units/mg protein (modified Warburg-Christian)
Synonym: GALT; UDP glucose:α-D-galactose-1-phosphate uridyltransferase
CAS Number: 9016-11-9
MDL Number: MFCD00131120
Product Type: Chemical
Galactose-1-phosphate uridyltransferase catalyzes the conversion of UDP-glucose to galactose-1-phosphate and galactose-1-phosphate to UDP-glucose.
| composition | Protein, 15-35% |
| foreign activity | 6-phosphogluconate dehydrogenase ≤0.5% |
| UDP glucose pyrophosphorylase and galactokinase ≤0.2% | |
| form | lyophilized powder |
| Quality Level | 300  |
| specific activity | 20-60 units/mg protein (modified Warburg-Christian) |
| storage temp. | −20°C |
| type | Type IV |
| Application: | Galactose-1-phosphate Uridyltransferase from galactose-adapted yeast has been used to perform enzyme assays. |
| General description: | Research area: Cell Signaling Galactose-1-phosphate uridyltransferase (GALT) is a galactose metabolizing enzyme that facilitates the simultaneous conversion of uridine diphosphoglucose (UDP-glucose) and galactose-1-phosphate (gal-1P) to uridine diphosphogalactose (UDP-galactose) and glucose-1-phosphate. Classic Galactosemia (CG) is an inherited metabolic condition caused by deficiency of GALT activity. GALT gene is mapped to human chromosome 9p13. Deficiency of GALT results in type 1 galactosemia. |
| Other Notes: | One unit will form 1.0 μmole of glucose 1-phosphate from UDP-glucose, galactose 1-phosphate and NADP+ per min at pH 8.7 at 25 °C as detected by a coupled system using phosphoglucomutase. |
| Packaging: | 20, 50 units in glass bottle |
| Physical form: | Contains buffer salts as citrate and reduced glutathione |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 20-60 units/mg protein (modified Warburg-Christian) |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.7.12 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |