β-Glucuronidase from Helix aspersa (garden snail)
SIGMA/G4259 - Type HA-4
EC Number: 232-606-8
MDL Number: MFCD00130629
Product Type: Chemical
β-Glucuronidase is routinely used to hydrolyze glucuronide-conjugates of drug metabolites in order to optimize sample preparation prior to LC-MS, GC and ELISA analysis.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G4259-1MU
| application(s) | clinical testing |
| form | partially purified powder |
| Quality Level | 200  |
| secondary activity | ≤7,500 units/g solid sulfatase |
| solubility | H2O: soluble 1.90-2.10 mg/mL, clear to slightly hazy |
| specific activity | ≥300,000 units/g solid solid |
| storage temp. | −20°C |
| type | Type HA-4 |
| Application: | β-glucuronidase was used in enzymic hydrolysis of tissue homogenates for liquid chromatography-electrospr |
| Biochem/physiol Actions: | β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption. |
| Other Notes: | One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37°C at the pH 5.0 (30 min assay). |
| Other Notes: | One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C. |
| Other Notes: | Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis |
| Packaging: | 1000000 units in poly bottle |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H317 - H334 |
| Precautionary statements | P261 - P280 - P342 + P311 |
| Hazard Codes | Xn |
| Risk Statements | 42/43 |
| Safety Statements | 22-36/37-45 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: ≥300,000 units/g solid solid; secondary activity: ≤7,500 units/g solid sulfatase |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 3.2.1.31 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |