Anti-Guanylyl Cyclase α1 antibody produced in rabbit
SIGMA/G4280 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-GC-S-alpha-1; Anti-GC-SA3; Anti-GCS-alpha-3; Anti-GUC1A3; Anti-GUCA3; Anti-GUCSA3; Anti-GUCY1A3; Anti-MYMY6
MDL Number: MFCD03455075
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Human heart sections stained with 1:1,000 Anti-Guanylyl Cyclase α1 antibody produced in Rabbit (Cat. No.G4280) (A). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 1:800 Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in Rabbit (Cat. No. G4405) (B). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-Guanylyl Cyclase antibodies, G4280 (A) and G4405 (B), target different regions of Guanylyl Cyclase show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin-fixed, paraffin-embedded section of bovine heart stained with 1:100 Anti-Guanylyl Cyclase α1 antibody produced in Rabbit (Cat. No. G4280) followed by Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Cytosolic fraction extract of Rat brain were separated on SDS-PAGE and probed with Anti-Guanylyl Cyclase α1 antibody produced in Rabbit (Cat. No. G4280). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A9169). Lanes 1. 1:10,000 antibody 2. 1:20,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | ~80 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | mouse, rat, human, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100 using trypsin-treated human, bovine and mouse heart tissue |
| immunoprecipitation (IP): 5-10 μg using 60-120 μg of a cytosolic fraction of rat brain | |
| western blot: 1:10,000 using cytosolic fraction of rat brain |
| Application: | Anti-Guanylyl Cyclase α1 antibody produced in rabbit is suitable for immunohistochemistry at a working dilution of 1:100 using trypsin-treated human, bovine and mouse heart tissue and immunoprecipitation at 5-10μg concentration using 60-120μg of a cytosolic fraction of rat brain. It is also suitable for western blot analysis using cytosolic fraction of rat brain at a working dilution of 1:10,000. It was used as a primary antibody for immunohistochemical: • localization of α1 subunits of sGC (soluble guanylate cyclase) in the guinea pig gastrointestinal tract • detection of expression of sGC in the vasculature of rat skeletal muscle • localization of the functional subunit of NO receptors, sGCα1 in guinea pig caecum |
| Biochem/physiol Actions: | Guanylyl cyclase (GC) catalyzes the conversion of guanosine-5′-triphosphate (GTP) to cyclic guanosine-3′,5-monophosph |
| Biochem/physiol Actions: | Guanylyl cyclase (GC) catalyzes the conversion of guanosine-5′-triphosphate (GTP) to cyclic guanosine-3′,5-monophosph |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The enzyme consists of α and β subunits of ~80kDa and ~70kDa respectively. Both the units are required for catalytic activity. The N-terminal domains of the subunits are essential for the stimulation of the enzyme by NO. Dimerization is mediated by the central portion of GC. The C-terminus domain of both subunits forms the catalytic domain. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 673-690 of rat soluble Guanylyl cyclase α1, conjugated to KLH with glutaraldehyde. The corresponding human sequence differs by 2 amino acids. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |