Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in rabbit
SIGMA/G4405 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-GC-S-beta-1; Anti-GC-SB3; Anti-GUC1B3; Anti-GUCB3; Anti-GUCSB3; Anti-GUCY1B3
MDL Number: MFCD03455078
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Human heart sections stained with 1:1,000 Anti-Guanylyl Cyclase α1 antibody produced in Rabbit (Cat. No.G4280) (A). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 1:800 Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in Rabbit (Cat. No. G4405) (B). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-Guanylyl Cyclase antibodies, G4280 (A) and G4405 (B), target different regions of Guanylyl Cyclase show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin fixed, paraffin-embedded Human heart tissue sections were stained with 1:800 Anti-Guanylyl Cyclase β-1 (ER-19) antibody produced in Rabbit (Cat. No. G4405) followed by 30 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 30 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886) Monoclonal Anti-Bovine IgG-Alkaline Phosphatase antibody produced in Mouse, Clone: BG-18 (Cat. No. A7554).
Western Blotting Rat brain extract were separated on SDS-PAGE and probed with Anti-Guanylyl Cyclase β-1 (ER-19) antibody produced in Rabbit (Cat. No. G4405). The antibody was developed using 1:5,000 Anti-Rabbit IgG (whole molecule)-Alkaline Phosphatase antibody produced in Goat (Cat. No. A9919). Lanes 1. 1:2,000 antibody 2. 1:4,000 antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 70 kDa |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, mouse, human, bovine |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:400 using trypsin-digested human, bovine and mouse heart tissue |
| immunoprecipitation (IP): 2-3 μg using 60-120 μg of a cytosolic fraction of rat brain | |
| western blot: 1:2,000 using cytosolic fraction of rat brain |
| Application: | Anti-Guanylyl Cyclase β1 (ER-19) antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:2000 using a cytosolic fraction of rat brain, immunoprecipitation at a working antibody amount of 2-3μg using 60-120μg of a cytosolic fraction of rat brain and for immunohistochemistry at 1:400 using trypsin-digested human, bovine and mouse heart tissue. It has been used as a primary antibody for: • localization of β1 subunits of sGC (soluble guanylate cyclase) in the guinea pig gastrointestinal tract • detection of expression of sGC in the vasculature of rat skeletal muscle • localization of the functional subunit of NO receptors, sGCβ1 in guinea pig caecum It has also been used in immunoblot assay to determine whether there were changes in uterine vascular smooth muscle (UVSM) sGC expression during the ovine reproductive cycle. |
| Biochem/physiol Actions: | Guanylyl cyclase (GC) catalyzes the conversion of guanosine-5’–triphosphate (GTP) to cyclic guanosine-3’,5-monophosph |
| Biochem/physiol Actions: | The enzyme (GC) is a major physiological receptor for nitric oxide (NO), an important intra- and intercellular membrane-permeant signaling molecule. Gaseous NO binds to Fe2+ in the prosthetic heme group of the enzyme. NO binding is followed by disruption of the β1 subunit histidine105 bond to iron and activation of the enzyme. GC forms a complex with NO and cGMP and regulates smooth muscle relaxation, inflammation, platelet adhesion and aggregation, pulmonary physiology and neuronal function. It is an important target for NO-releasing and non-NO-releasing activator drugs in human cardiovascular therapy. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Soluble guanylyl cyclase (sGC) is an obligate hemoprotein enzyme consisting of α and β subunits of ~80 kDa and ~70 kDa, respectively. |
| Immunogen: | synthetic peptide corresponding to amino acid residues 189-207, with N-terminal added lysine, of rat soluble guanylate cyclase 1, conjugated to KLH with glutaraldehyde. The sequence differs in human, bovine and mouse by 1 amino acid. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |