Anti-GABAA Receptor (α1 subunit) antibody produced in rabbit
SIGMA/G4416 - affinity isolated antibody
MDL Number: MFCD01092338
Product Type: Chemical
Immunohistochemistry Anti-GABA(A) α1 Receptor (extracellular): Cat. No. G4416: Immunohistochemical staining of Mouse hippocampus using Anti-GABA(A) α1 Receptor (extracellular) antibody. A. Distribution of GABA(A) α1 Receptor (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that distribution of GABA(A) α1 Receptor is restricted to neurons and their processes. DAPI is used as the counterstain (blue).
Immunohistochemistry Anti-GABA(A) α1 Receptor (extracellular): Cat. No. G4416: Immunohistochemical staining of Rat cerebellum using Anti-Cav1.2 antibody and Anti-GABA(A) α1 Receptor (extracellular) antibody. A. Cav1.2 (green) is detected in the granule layer of the cerebellum (G) and in the upper molecular layer (star). B. In the same section, GABA(A) α1 Receptor (red) is seen in the granule layer. C. Merge of the two images reveals high degree of colocalization between Cav1.2 and GABA(A) α1 Receptor in the granule layer. Immunohistochemical staining of Mouse hippocampus using Anti-GABA(A) α1 Receptor (extracellular) antibody. A. Distribution of GABA(A) α1 Receptor (red). B. Distribution of glial fibrillary acidic protein (green). C. Merge of the two images indicates that distribution of GABA(A) α1 Receptor is restricted to neurons and their processes. DAPI is used as the counterstain (blue).
Immunocytochemistry Anti-GABA(A) α1 Receptor (extracellular): Cat. No. G4416: Immunocytochemical staining of intact living Rat pheochromocytoma PC12 cells. A. Extracellular staining of cells using Anti-GABA(A) α1 Receptor (extracellular) antibody, (1:100) followed by Goat Anti-Rabbit-AlexaFluor-594 secondary antibody. B. Merge image of A and live view of the cells.
Immunoblotting
Anti-GABA(A) α1 Receptor (extracellular): Cat. No. G4416: Western blot analysis of Rat brain lysates.
Lanes
1. Anti-GABA(A) α1 Receptor (extracellular) antibody, (1:1,000).
2. Anti-GABA (A) α1 Receptor (extracellular) antibody preincubated with the control peptide antigen.
Anti-GABA(A) α1 Receptor (extracellular): Cat. No. G4416: Western blot analysis of Rat brain lysates.
Lanes
1. Anti-GABA(A) α1 Receptor (extracellular) antibody, (1:1,000).
2. Anti-GABA (A) α1 Receptor (extracellular) antibody preincubated with the control peptide antigen.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | lyophilized powder |
| mol wt | antigen ~50 kDa |
| Quality Level | 100  |
| species reactivity | mouse, rat, human (predicted), bovine (predicted), chicken (predicted) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: suitable using rat brain sections |
| western blot: 1:400-1:1,000 using rat brain membranes | |
| UniProt accession no. | P14867  |
| Application: | Anti-GABAA Receptor (α1 subunit) antibody produced in rabbit is suitable for detection of GABA-A α1 subunit by immunohistochemistry using rat brain sections and by immunoblotting at a working dilution of 1:400-1:1000 using rat brain membranes. |
| Biochem/physiol Actions: | The inhibitory neurotransmitter GABA signals through two distinct types of pre- and postsynaptic receptors, GABAA and GABAB. Both GABA receptors can regulate depression of synaptic transmission and be involved in the inhibition controlling neuronal excitability. α1 is a subtype of GABAA receptor expressed selectively by serotonergic and GABAergic neurons. Serotonergic neurons express strong α3 immunoreactivity but, do not show any α1 immunoreactivity. On the contrary, GABAergic neurons express both α1 and α3 subunits. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GABAA and GABAB receptors differ with regard to their ionic characteristics and pharmacological properties. The GABAA receptor is an ionotropic receptor that forms the GABA gated chloride channel and consists of several heterogeneous subunits with membrane recognition sites for benzodiazapenes. |
| Immunogen: | peptide corresponding to amino acids 28-43 of rat GABA(A) receptor α1 subunit. |
| Physical form: | Lyophilized from phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 0.05% sodium azide. |
| Preparation Note: | Reconstitute the 0.05 mL vial with 50 μL deionized water. Reconstitute the 0.2 mL vial with 0.2 mL deionized water. After reconstitution, the antibody concentration is ~0.8 mg/mL. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |