Anti-GRP94 (C-terminal) antibody produced in rabbit
SIGMA/G4420 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Endoplasmin precursor; Anti-Glucose Regulated Protein 94; Anti-Tumor Rejection Antigen 1; Anti-gp96
MDL Number: MFCD01322081
Product Type: Chemical
Western Blotting Whole extract of NIH-3T3 Mouse fibroblasts cells were separated on SDS-PAGE and probed with Anti-GRP94 (C-terminal) antibody produced in Rabbit (Cat. No. G4420). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1 μg/mL antibody 2. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 94 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | canine, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunoprecipitation (IP): 10-20 μg using MDCK whole cell lysate | |
| indirect immunofluorescence: 2-4 μg/mL using HeLa cells | |
| microarray: suitable | |
| western blot: 0.5-1 μg/mL using whole cell extracts of HeLa, MDCK, and NIH3T3 cells | |
| UniProt accession no. | P14625  |
| Application: | Anti-GRP94 (C-terminal) antibody produced in rabbit has been used in immunocytochemistry. |
| Application: | Anti-GRP94 (C-terminal) antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5-1μg/mL using whole cell extracts of the human epitheloid carcinoma HeLa cells, Madin Darby canine kidney (MDCK) cells, and mouse fibroblast NIH3T3 cells. It is also suitable for immunoprecipitation of GRP94 protein from a MDCK whole cell lysate at 10-20μg concentration. |
| Biochem/physiol Actions: | GRP94 (glucose-regulated protein 94) is involved in protein folding, sorting and secretion, and binding of immunogenic peptides. GRP94 has a role in peptide antigen presentation to major histocompatibility complex class I molecules of antigen presenting cells (APCs). It binds specifically to CD91 (α2-macroglobulin receptor), and possibly to the Toll-like receptors (TLRs) on the surface of APCs. Overexpression of GRP94 has been associated with cellular transformation and tumorigenesis. |
| Biochem/physiol Actions: | GRPs are unresponsive to heat stress and are induced by stress related to glucose starvation or defects in glycoprotein processing, depletion of Ca2+ stores, acidosis, and hypoxia conditions that are also common in poorly vascularized tumor tissues. |
| Biochem/physiol Actions: | The GRP94 (glucose-regulated protein 94) is a 94 kDa Ca2+- binding glycoprotein that belongs to a subfamily of the heat shock proteins Hsp90. It is a chaperone protein constitutively localized to the endoplasmic reticulum (ER) of mammalian cells. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | synthetic peptide corresponding to amino acids 733-750 located near the C-terminus of human GRP94. This sequence is identical in mouse, rat, dog, porcine, and bovine GRP94 and highly conserved (1 amino acid substitutuion) in chicken. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |