Glycerokinase from Escherichia coli
SIGMA/G4509 - lyophilized powder, 40-100 units/mg protein
Synonym: ATP:glycerol 3-phosphotransferase; Glycerol Kinase
CAS Number: 9030-66-4
EC Number: 232-862-0
MDL Number: MFCD00066766
Product Type: Chemical
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| biological source | Escherichia coli |
| composition | Protein, ≥50% biuret |
| foreign activity | Triose-phosphate isomerase, hexokinase, myokinase, glycerol dehydrogenase and NADH oxidase ≤0.05% |
| form | lyophilized powder |
| Quality Level | 200  |
| specific activity | 40-100 units/mg protein |
| storage temp. | −20°C |
| Application: | Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis. |
| Biochem/physiol Actions: | Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. <<<20,21>>> It is also subject to feedback regulation by fructose-1,6-bisphosphate |
| Biochem/physiol Actions: | Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate |
| Other Notes: | One unit will convert 1.0 μmole of glycerol and ATP to |
| Packaging: | 1000 units in serum bottle |
| Physical form: | Partially purified lyophilized powder, balance is primarily salts and EDTA. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 40-100 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.1.30 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |