Glutathione−Agarose
SIGMA/G4510 - lyophilized powder
Synonym: Glutathione-Agarose Resin; GSH-agarose; S-linked glutathione agarose
MDL Number: MFCD00131200
Product Type: Chemical
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| analyte chemical class(es) | proteins (GST) |
| extent of labeling | ≥10 μmol per mL gel |
| form | lyophilized powder |
| matrix | 4% cross-linked beaded agarose |
| matrix activation | epoxy |
| matrix attachment | sulfur |
| matrix spacer | 12 atoms (10 carbon) |
| Quality Level | 200  |
| storage temp. | −20°C |
| technique(s) | immunoprecipitation (IP): suitable |
| protein purification: suitable |
| Application: | Affinity chromatography using glutathione-agarose permits rapid, mild, non-denaturing and highly selective purification of proteins containing glutathione binding sequences, such as Glutathione S-Transferase (GST), glutathione peroxidase and glyoxalase I. |
| Application: | Glutathione-Agarose has been used: • to purify glutathione S-transferase (GST)-fused protein for generating polyclonal antibody M4P • to incubate supernatant fractions for the purification of full-length p53 (aa 1-393) and its DNA-binding domain (aa 94-288) • in affinity purification of the fusion protein |
| Biochem/physiol Actions: | Glutathione S-transferases (GSTs), a superfamily of multifunctional detoxification isoenzymes, have a significant role in cellular signaling. GSTs are emerging as promising therapeutic targets due to the overexpression of specific isozymes in various tumors and their potential involvement in the development of other diseases such as neurodegenerative diseases, multiple sclerosis, and asthma. |
| Features and Benefits: | • Offers rapid, mild, non-denaturing, and highly selective purification of glutathione-binding enzymes. • Has a shelf life of two years if stored properly desiccated at -20 °C. • Binding capacity: 5-10 mg glutathione S-transferase per mL resin |
| General description: | Glutathione S-transferase (GST) is a protein with a molecular weight of 26 KDa that naturally occurs in eukaryotic cells. It belongs to the family of Phase II detoxification enzymes. Glutathione-Agarose consists of glutathione attached through its sulfur to epoxy-activated 4% cross-linked beaded agarose, resulting in a 12-atom (10-carbon) spacer. It is provided as a lyophilized powder stabilized with lactose. |
| Packaging: | 1, 5, 10, 50 mL in poly bottle |
| Physical form: | Lyophilized powder stabilized with lactose |
| Preparation Note: | One g powder swells to ≥10 ml of gel |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 41106500 |