Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit
SIGMA/G4546 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: GFAP Antibody for Flow Cytometry - Anti-Glial Fibrillary Acidic Protein antibody produced in rabbit; Gfap Antibody Flow Cytometry; Anti-FLJ45472; Anti-GFAP; Anti-Intermediate filament protein
MDL Number: MFCD00145904
Product Type: Chemical
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| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, rat, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 1:200-1:500 |
| immunohistochemistry: 1:100-1:200 | |
| western blot: 1:500-1:1,000 | |
| UniProt accession no. | P14136  |
| Application: | Rabbit polyclonal anti-GFAP antibody is used to tag glial fibrillary acidic protein for detection and quantitation by Western blotting and immunohistochemical (IHC) techniques. It is used as a probe to determine the roles of glial fibrillary acidic protein in cell:cell communications, mitosis and cell structure. Anti-GFAP has proven a valuable tool for use in immunocytochemical localization of GFAP in normal central nervous system tissue, certain tumors and metastases of the glial antigen, as well as for immunofluorescent labeling of cultured mammalian cells. |
| Biochem/physiol Actions: | Rabbit polyclonal anti-Glial Fibrillary Acidic Protein recognizes human, mouse, and rat GFAPs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Intermediate filaments (IFs) with characteristic 10 nm diameter are a distinct class of molecularly heterogenous cytoskeletal filaments defined by ultrastructural, immunological, and biochemical criteria. Intermediate filaments differ significantly from the other cytoskeletal elements of the cell, namely microtubules and microfilaments, and are components of most eukaryotic cells. Glial Fibrillary Acidic Protein (GFAP) (molecular weight of 50 kDa) is found in astrocytes and ependymal cells of the central nervous system. It is found in various other cell types including stellate cells, chondrocytes, osteocytes, keratinocytes, Leydig cells and fibroblasts. GFAP is involved in mitosis, and cell:cell interactions and communications. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. |
| Immunogen: | synthetic peptide corresponding to an internal C-terminal sequence of human GFAP. |
| Physical form: | solution in phosphate buffered saline, containing 0.02% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |