Granulocyte-Macrophage Colony-Stimulating Factor human
SIGMA/G5035 - GM-CSF, recombinant, expressed in E. coli, suitable for cell culture
Synonym: GM-CSF human
MDL Number: MFCD00166611
Product Type: Chemical
SDS-PAGE Granulocyte-Macrophage Colony-Stimulating Factor Human (Cat. No. G5035) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of GM-CSF that elicits a 50% increase of Alkaline Phosphatase in cell based bioassay. Cell line: TF1.
| assay | ≥98% (SDS-PAGE) |
| biological source | human |
| color | white |
| form | lyophilized powder |
| impurities | ≤1.000 EU/μg |
| mol wt | 14.6 kDa |
| packaging | pkg of 4X5 μg |
| pkg of 5 μg | |
| potency | ≤0.1 ng/mL EC50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| solubility | water: soluble 0.010 mL, clear, colorless |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | P04141  |
| Analysis Note: | The proliferative activity of human GM-CSF is tested in culture using human TF-1 cells. |
| Biochem/physiol Actions: | Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity. |
| General description: | Package size based on protein content |
| Physical form: | Lyophilized from a 0.2 μm filtered, 10 mM sodium citrate, pH 3.5 solution. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |