Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in rabbit
SIGMA/G5038 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-GAD 65
MDL Number: MFCD03455043
Product Type: Chemical
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Rat Pancreas sections stained with 1:1,000 Anti-Glutamic Acid Decarboxylase 65/67 antibody produced in Rabbit (Cat. No.G5163) (A). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 1:1,000 Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit (Cat. No. G5038) (B). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-Glutamic Acid Decarboxylase 65/67 antibodies, G5163 (A) and G5038 (B), target different regions of Glutamic Acid Decarboxylase 65/67 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Formalin fixed, paraffin-embedded Rat pancreas tissue sections were stained with 1:1,000 Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit (Cat. No. G5038) followed by 20 μg/mL Anti-Rabbit IgG (whole molecule)-Biotin antibody produced in Goat (Cat. No. B8895) and by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886).
Western Blotting Whole extract of Rat brain were separated on SDS-PAGE and probed with Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit (Cat. No. G5038). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:4,000 antibody 2. 1:8,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | human, mouse, rat, pig, monkey |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using tissue sections of rat pancreas. |
| microarray: suitable | |
| western blot: 1:4,000 using rat brain extract | |
| UniProt accession no. | Q05329  |
| Application: | Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in rabbit has been used in immunohistochemistry and immunocytochemistry. |
| Biochem/physiol Actions: | Glutamic Acid Decarboxylase (GAD) catalyzes the conversion of L-glutamate to γ-aminobutyric acid (GABA), the principal inhibitory neurotransmitter in the brain, and a putative paracrine signal molecule in pancreatic islets. GAD65 is an ampiphilic, membrane-anchored protein, (585 amino acid residues) and is encoded on human chromosome 10. It has been identified as an autoantigen in insulin-dependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS) and may serve as a marker in the early stages of IDDM. GAD 65 synthesizes transmitter GABA for vesicular release. |
| Biochem/physiol Actions: | Reacts specifically with GAD 65 (65 kDa). |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GAD is predominantly expressed in the central nervous system (CNS) and pancreatic islets. It is also expressed in testis, oviduct and ovary. GAD exists in two isofoms, GAD 65 and GAD 67. GAD65 is targeted to membranes and nerve endings. |
| Immunogen: | synthetic peptide corresponding to the N-terminal region of human GAD 65 (amino acids 5-22). The sequence is highly conserved in rat and pig GAD 65 (single amino acid substitution) and mouse and monkey GAD 65, but is not found in GAD 67. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |