Sephadex® G-50
SIGMA/G5080 - Fine
Synonym: Gel filtration resin; Sephadex G-50 Fine Medium
CAS Number: 9048-71-9
MDL Number: MFCD00132771
Product Type: Chemical
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G5080-50G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G5080-10G
| application(s) | life science and biopharma |
| bead size | 20-80 μm |
| compatibility | Cytiva |
| matrix active group | phase |
| Quality Level | 200  |
| swelling | 1 g swells to 9-11 mL |
| technique(s) | affinity chromatography: suitable |
| Application: | Sephadex® G-50 is suitable for use in: • the separation of low and high molecular weight molecules • affinity chromatography, protein chromatography, and gel filtration chromatography |
| Application: | Sephadex® G-50 has been used: • to remove the non-entrapped carboxyfluorescein (CF) from the liposome suspension • in the purification of monoclonal antibody (mAb) humanized MN-14 by centrifuged size-exclusion chromatography • in desalting the recombinant enzymes eluted in the fraction five and six |
| Features and Benefits: | • Desalting with Sephadex is considered superior to dialysis because it saves time, has a low dilution factor, and recovers high activity even with minute amounts of sample |
| Features and Benefits: | • Desalts, removes contaminants, and transfers to a new buffer in one step. • Suitable for DNA purification from small molecules using gel filtration. • Features a small bead size, resulting in shorter diffusion distances. • Considered a classic gel filtration resin. |
| General description: | Sephadex G-50 Fine is a widely used gel filtration resin designed for desalting and buffer exchange of biomolecules with a molecular weight greater than 30,000. Its small bead size contributes to higher efficiency in these processes. Different types of Sephadex vary in terms of cross-linking, resulting in differences in swelling and molecular fractionation range. Sephadex G-50 is one of five G-types available, ranging from G-10 for small molecules to G-75 for larger molecules. Sephadex G-50 comes in four different particle sizes, such as Coarse, Medium, Fine, and Superfine. |
| General description: | Sephadex® G-50 is a gel filtration medium used in affinity chromatography, protein chromatography, and gel filtration chromatography. Sephadex is a beaded gel prepared by crosslinking dextran with epichlorohydrin. Fractionation Range (MW) Globular Proteins: 1,500 - 30,000 Dextrans: 500 - 10,000 |
| Legal Information: | Sephadex is a registered trademark of Cytiva |
| Other Notes: | G5080-100G′s updated product number is GE17-0042-01 G5080-500G′s updated product number is GE17-0042-02 |
| Packaging: | 10, 50, 100, 500 g in poly bottle |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| UNSPSC | 23151817 |