Anti-Glutamic Acid Decarboxylase 65/67 antibody produced in rabbit
SIGMA/G5163 - IgG fraction of antiserum, buffered aqueous solution
Synonym: Anti-GAD 65/67
MDL Number: MFCD01322096
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Nicotinic receptor Alpha7 expression during mouse adrenal gland development. By: Gahring, L. C., Myers, E., et al. in PLoS One, 2014. PubMed ID: 25093893 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0103861), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Enhanced Validation-By Independent Antibodies Immunohistochemistry. Formalin-fixed, paraffin-embedded Rat Pancreas sections stained with 1:1,000 Anti-Glutamic Acid Decarboxylase 65/67 antibody produced in Rabbit (Cat. No.G5163) (A). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886), and 1:1,000 Anti-Glutamic Acid Decarboxylase 65 (5-22) antibody produced in Rabbit (Cat. No. G5038) (B). The antibody was developed using 20 μg/mL Goat Anti-Rabbit IgG (whole molecule)-Biotin (Cat. No. B8895) followed by 20 μg/mL ExtrAvidin®-Peroxidase (Cat. No. E2886). Results Two Anti-Glutamic Acid Decarboxylase 65/67 antibodies, G5163 (A) and G5038 (B), target different regions of Glutamic Acid Decarboxylase 65/67 show similar staining profiles between the two antibodies, demonstrating Independent Antibody Verification.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded Rat pancreas sections with 1:1,000 Rabbit Anti-Glutamic Acid Decarboxylase 65/67 (Cat. No. G5163) using biotin/ExtrAvidin®-Peroxidase.
Western Blotting Whole extract of Rat Brain were separated on SDS-PAGE and probed with Anti-Glutamic Acid Decarboxylase 65/67 antibody produced in Rabbit (Cat. No. G5163). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 1:10,000 antibody 2. 1:20,000 antibody 3. Negative Control
| antibody form | IgG fraction of antiserum |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| enhanced validation | independent Learn more about Antibody Enhanced Validation  |
| form | buffered aqueous solution |
| mol wt | antigen 65-67 kDa |
| packaging | antibody small pack of 25 μL |
| Quality Level | 200 |
| shipped in | dry ice |
| species reactivity | rat, mouse, feline, pig, human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using tissue sections of rat pancreas |
| microarray: suitable | |
| western blot: 1:10,000 using rat brain extract | |
| UniProt accession no. | Q05329  |
| Application: | Anti-GAD 65/67 may be used for the detection and localization of GAD 65/67 isoforms by immunofluorescence and immunohistochemistry in rat retinal cells, human subthalamus neurons, and pancreatic β-cells of rat. The minimum working dilution that may be used is 1:10000. Detection by immunoblotting may be possible in brain cells of monkey, human, rat and gecko. at a minimum working dilution of 1:1000. |
| Application: | Anti-Glutamic Acid Decarboxylase 65/67 antibody produced in rabbit has also been used in Immunohistochemistry and Immunocytochemistry. |
| Biochem/physiol Actions: | Glutamic acid decarboxylase (GAD) is the key enzyme that catalyses the conversion of |
| Biochem/physiol Actions: | Reacts specifically with GAD 65 and GAD 67 isoforms from brain. |
| Biochem/physiol Actions: | Reacts specifically with GAD 65 and GAD 67 isoforms in extracts of rat brain and rat pancreas. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GAD 65 has been identified as an autoantigen in insulin dependent diabetes mellitus (IDDM) and stiff-man syndrome (SMS). |
| General description: | GAD is highly expressed in GABAergic neurons and pancreatic β cells. It is transiently expressed in non-GABAergic embryonic and adult neurons, indicating a role in development. It is also present in testis, oviduct, and ovary GAD exists as two isoforms, GAD 65 and GAD 67 (molecular masses of 65 and 67 kDa, respectively) that are encoded by two different genes. GAD65 is an ampiphilic, membrane anchored protein, (585 amino acid residues) and is encoded on human chromosome 10. GAD 67 is a cytoplasmic protein (594 amino acid residues) and is encoded on chromosome 2. There is 64% amino acid idENTITY_TYPE_CODE. The two isoforms appear to have distinct intraneuronal distribution in the brain. The protein is initially localized to cytosol and then gets anchored to the membrane upon post translational modification. The GAD2 gene is mapped to human chromosome 10p12.1. |
| Immunogen: | synthetic peptide corresponding to the C-terminal region of human GAD 67 (amino acids 579-594). The sequence is identical in human GAD 65 (amino acids 570-585), in rat, mouse, and pig GAD 65, in rat, pig, and cat GAD 67, and is highly conserved in GAD 67 of mouse origin (single amino acid substitution). |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |