Monoclonal Anti-Glutamic Acid Decarboxylase 67 (GAD67) antibody produced in mouse
SIGMA/G5419 - clone K-87, purified antibody
Synonym: Anti-GAD 67
MDL Number: MFCD06202147
Product Type: Chemical
Immunohistochemical staining of GAD67 in somata and terminals of the rat cerebral cortex using Monoclonal Anti-GAD67 (Cat. No. G5419). Note that this antibody stains both somata and terminals after Triton X detergent treatment. 4% paraformaldehyde fixation/Vibratome sections.
Immunohistochemistry
Fixed mouse brain sections underwent antigen retrieval and were stained with Monoclonal Anti-GAD 67, clone K-87 (Cat No. G5419) at 1:2,000. Cerebellum is shown.
Fixed mouse brain sections underwent antigen retrieval and were stained with Monoclonal Anti-GAD 67, clone K-87 (Cat No. G5419) at 1:2,000. Cerebellum is shown.
| antibody form | purified antibody |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | K-87, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| isotype | IgG1κ |
| mol wt | antigen 67 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, mouse, human |
| storage temp. | −70°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500-1:20,000 (with antigen-retrieval step) |
| western blot: 1:500-1:20,000 using mouse brain extract | |
| UniProt accession no. | Q99259  |
| Application: | Monoclonal Anti-Glutamic Acid Decarboxylase 67 (GAD67) antibody is suitable for use in immunoblot, western blot (at 1:500-1:20,000 dilutions using mouse brain extract) and immunohistochemistry (at 1:500-1:20,000 dilutions in mouse brain sections). |
| Application: | Monoclonal anti-glutamic acid decarboxylase 67 (GAD67) antibody produced in mouse has been used in: • immunohistofluorescence • western blotting • immunohistochemistry. |
| Biochem/physiol Actions: | Glutamic Acid Decarboxylase 67 (GAD67) is involved in the production of γ-amino butyric acid (GABA), a inhibitory neurotransmitter in the central nervous system (CNS). Deficiency of GAD67 leads to psychosis, schizophrenia and bipolar diseases. |
| Biochem/physiol Actions: | Reacts specifically with GAD67. Does not crossreact with GAD65. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Glutamic Acid Decarboxylase 67 (GAD67) is encoded by the gene glutamic acid decarboxylase 1 (GAD1). GAD1 gene is located on the human chromosome at 2q31.1. GAD67 is localized in the dendrites and cell bodies. |
| Immunogen: | synthetic peptide from mouse GAD67 (amino acids 87-106). |
| Immunogen: | This peptide does not share homology with GAD65. |
| Physical form: | Solution in phosphate buffered saline containing 0.01% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −70°C |
| UNSPSC | 12352203 |