Anti-β-Glucuronidase (N-Terminal) antibody produced in rabbit
SIGMA/G5420 - ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-GUS
MDL Number: MFCD00282142
Product Type: Chemical
Western Blotting Purified β-Glucuronidase from E.coli (Cat. No. G7646) were separated on SDS-PAGE and probed with Anti-β-Glucuronidase (N-Terminal) antibody produced in Rabbit (Cat. No. G5420). The antibody was developed using 1:10,000 Anti-Rabbit IgG (whole molecule)-Peroxidase antibody produced in Goat (Cat. No. A0545). Lanes 1. 0.5 μg/mL antibody 2. 1 μg/mL antibody 3. Negative Control
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.5 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 60 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | plant |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | western blot: 0.5-1 μg/mL using GUS purified from E. coli |
| Application: | Anti-β-Glucuronidase (N-Terminal) antibody produced in rabbit has been used in western blotting. |
| Application: | Rabbit polyclonal anti-β-Glucuronidase (N-Terminal) antibody may be used for the detection of GUS by immunoblotting (60 kDa). It may be used to detect the reporter GUS marker protein in transgenic plants. |
| Biochem/physiol Actions: | β-Glucuronidase (GUS) is a screenable markers E. coli GUS has been extensively used to monitor transgene delivery to plant tissue. |
| Biochem/physiol Actions: | β-Glucuronidase catalyzes the cleavage of terminal glucuronic acid bound by β-linkage from mono-, oligo-, or polysaccharides or phenols. GUS is mainly used as a marker during transgenic events. In vitro GUS activity assays predominantly facilitates histochemical and fluorometric localization in tissues. GUS activity is also used for the analysis of expression of foreign genes. GUS reporter genes fused with specific promoter genes have been used for the determination of transformed tissues on culture media containing antibiotics. GUS-specific antibodies are helpful in detecting GUS gene product in transformed plants. |
| Biochem/physiol Actions: | Anti-β-Glucuronidase (N-Terminal) recognizes bacterial GUS expressed in transgenic tobacco plants. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | β-Glucuronidase (GUS) is encoded by the E. coli gus gene (also referred to as uidA), GUS protein (60 kDa) is an hydrolase. |
| Immunogen: | Sythetic peptide corresponding to amino acids located at the N-terminus of E. coli GUS, conjugated to KLH. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frostfree” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |