Giemsa stain
SIGMA/G5637 - certified by the BSC
Synonym: Azure mixture sicc. Giemsa stain
CAS Number: 51811-82-6
Empirical Formula (Hill Notation): C14H14ClN3S
Molecular Weight: 291.80
MDL Number: MFCD00081642
Linear Formula: C14H14ClN3S
Product Type: Chemical
ATR-IR Spectra for Azure mixture sicc. Giemsa stain.
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G5637-25G
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G5637-5G
| absorption | ≥0.6 at 647-653 nm in methanol at 0.005 g/L |
| agency | certified by the BSC |
| application(s) | diagnostic assay manufacturing hematology histology |
| color | dark green to very dark green |
| form | powder or crystals |
| InChI | 1S/C14H13N3S.ClH/c1-17(2) |
| InChI key | NALREUIWICQLPS-UHFFFAOYSA |
| mp | 300 °C (lit.) |
| Quality Level | 200  |
| SMILES string | [Cl-].CN(C)c1ccc2N=C3C=CC |
| solubility | ethanol: 1 mg/mL, blue to very deep blue |
| storage temp. | room temp |
| technique(s) | microbe id | staining: suitable |
| Application: | Certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections. |
| Application: | Giemsa stain has been used for the staining of cells and blood films.It is required for the staining of azurophil granules and is an excellent stain for inclusion bodies.Its applications can be seen in hematology for blood and bone marrow specimens, bacteriology, clinical cytology specimens, histological biopsies, and tumor samples. |
| General description: | Giemsa is a versatile polychromatic stain, which is suitable for staining a diverse range of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites. It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa. The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. Giemsa′s staining solution is composed of methylene blue, azure, and eosin. |
| Other Notes: | Nuclei will be varying shades of purple. Cytoplasmic staining will be varying shades of blue to light pink. Fine reddish to lilac granules may be present in cytoplasm of some cell types. Basophils will demonstrate dark blue black granules in the cytoplasm. Eosinophils will demonstrate bright orange granules in the cytoplasm. Red blood cells should be pink to orange |
| Packaging: | 5, 25 g in glass bottle |
| Hazard Codes | Xn |
| Risk Statements | 22-41 |
| Safety Statements | 26-39 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| mp | 300 °C (lit.) |
| Storage Temp. | room temp |
| UNSPSC | 12171500 |