Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
SIGMA/G5760 - Type XXIII, ammonium sulfate suspension, 550-1,100 units/mg protein (biuret), ≥2.0 mg/mL Biuret
Synonym: G-6-P-DH
CAS Number: 9001-40-5
EC Number: 232-602-6
MDL Number: MFCD00081656
Product Type: Chemical
Mechanism of Glucose-6-phosphate Dehydrogenase D-glucose 6-phosphate + NADP+ +H2O→ 6-phospho-D-gluconate + NADPH + H+
| biological source | bacterial (Leuconostoc mesenteroides) |
| concentration | ≥2.0 mg/mL Biuret |
| foreign activity | 6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005% |
| PGI ≤0.01% | |
| form | ammonium sulfate suspension |
| mol wt | 54 kDa |
| Quality Level | 200  |
| specific activity | 550-1,100 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| type | Type XXIII |
| Application: | Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used:
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| Biochem/physiol Actions: | Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway. |
| Biochem/physiol Actions: | Glucose-6-phosphate dehydrogenase (G6PD) is a housekeeping enzyme that protects cells from damage caused by reactive oxygen species by supplying substrates that help prevent oxidative harm. G6PD from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+) as the first step in the pentose phosphate pathway. Lysine 21 in the Glucose-6-phosphate Dehydrogenase (G6PD) is crucial for binding NAD+. G6PD uses NAD+ or NADP+ based on the catabolic or anabolic metabolic pathway.G6PD deficiency can lead to acute hemolysis, neonatal jaundice, or severe chronic non-spherocytic hemolytic anemia. |
| General description: | Research area: Cell Signaling Glucose-6-phosphate dehydrogenase (G6PD) is found in the cytoplasm of all cells. The G6PD enzyme is encoded by the Gd gene, located on the long arm of the X chromosome.Glucose-6-phosphate Dehydrogenase (G6PD) from Leuconostoc mesenteroides catalyzes the oxidation of glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide (NAD+) or nicotinamide adenine dinucleotide phosphate (NADP+). It corresponds to a molecular weight of 54 kDa and exists as a homodimer. |
| Other Notes: | One unit will oxidize 1.0 μmole of |
| Physical form: | Suspension in 2.7 M (NH4)2SO4 solution containing 42 mM Tris and 0.8 mM MgCl2 |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 550-1,100 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.49 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |