Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides
SIGMA/G5885 - lyophilized powder, >= 550 units/mg protein (biuret)
Synonym: G-6-P-DH
CAS Number: 9001-40-5
EC Number: 232-602-6
MDL Number: MFCD00081656
Product Type: Chemical
Mechanism of Glucose-6-phosphate Dehydrogenase D-glucose 6-phosphate + NADP+ +H2O→ 6-phospho-D-gluconate + NADPH + H+
| application(s) | agriculture |
| biological source | bacterial (Leuconostoc mesenteroides) |
| composition | Protein, 15-40% biuret |
| foreign activity | 6-Phosphogluconic dehydrogenase, hexokinase, NADH oxidase and NADPH oxidase ≤0.005% |
| PGI ≤0.01% | |
| form | lyophilized powder |
| mol wt | 128 kDa |
| Quality Level | 300  |
| specific activity | >= 550 units/mg protein (biuret) |
| storage temp. | 2-8°C |
| type | Type XXIV |
| Application: | Glucose-6-phosphate Dehydrogenase from Leuconostoc mesenteroides has been used along with hexokinase in the determination of glucose from mice liver samples. |
| Biochem/physiol Actions: | Glucose-6-Phophate Dehydrogenase (G-6-PDH) can utilize either nicotinamide adenine dinucleotide phosphate (NADP+) or NAD+ as coenzyme making it crucial for bacterial metabolism. |
| Biochem/physiol Actions: | Glucose-6-phosphate dehydrogenase (G6PD) catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway. |
| General description: | Glucose-6-Phophate Dehydrogenase (G-6-PDH) comprises His-Asp catalytic dyad and exists as a homodimer. Structurally, G-6-PDH encompasses a Rossmann dinucleotide binding fold in the coenzyme binding domain region. It also harbors a large β + α domain and has a unique aspartate residue at position 374. |
| Other Notes: | One unit will oxidize 1.0 μmole of |
| Packaging: | 10000 units in glass bottle |
| Packaging: | 200, 1000, 2000 units in serum bottle |
| Physical form: | Lyophilized powder containing Ficoll and Tris buffer salts |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P304 + P340 + P312 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: >= 550 units/mg protein (biuret) |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 1.1.1.49 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |