Gamborg’s B-5 Basal Medium with Minimal Organics
SIGMA/G5893 - fine powder, suitable for plant cell culture
Synonym: B-5 Basal Medium; Basal Medium; Minimal Organics Medium
Product Type: Chemical
| application(s) | agriculture |
| form | fine powder |
| Quality Level | 200 ![]() |
| storage temp. | 2-8°C |
| technique(s) | cell culture | plant: suitable |
| Application: | Gamborg′s B-5 Basal Medium with Minimal Organics has also been used: • as a component of liquid media for the tissue culture of Brassica rapa • as a component of callus inducing medium for culturing Arabidopsis cardiolipin mutant seedlings • as a component of solid GM medium for germination of Arabidopsis seeds |
| General description: | Gamborg’ B-5 medium may be purchased as a complete medium (G5893) or reconstituted from Vitamin 1000x (G1019) and Basal Salt Mixture (G5768) chemstocks. Use Gamborg’s B-5 (B5) medium for in vitro culture of plant cell lines and tissues such as of Taxus globosa, Splachnum ampullaceum Hedw, Linum album, Eucalyptus camaldulensis. Gamborg’s B-5 medium has been used for the culture of Annona squamosa callus. |
| General description: | Gamborg′s B-5 basal medium is an efficient nutrient mixture for plants. |
| Preparation Note: | Formulated to contain 3.2 grams of powder per liter of medium. |
| Preparation Note: | With the macro- and micronutrients, and vitamins as described by Gamborg, et al. (1968). Media Formulation ![]() |
| Symbol | GHS03 |
| Signal word | Warning |
| Hazard statements | H272 |
| Precautionary statements | P210 - P220 - P280 - P370 + P378 - P501 |
| Hazard Codes | O |
| Risk Statements | 8 |
| RIDADR | UN 1486 5.1 / PGIII |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352207 |


