Anti-GW182 antibody produced in rabbit
SIGMA/G5922 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-TNRC6A; Anti-Trinucleotide repeat containing 6A
Product Type: Chemical
Immunofluorescence Human epithelial HEp-2 cells were stained with 5 μg/mL Rabbit Anti-GW182 (Cat. No. G5922). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2.5-5.0 μg/mL using human epithelial HEp-2 cells |
| UniProt accession no. | Q8NDV7  |
| Application: | Anti-GW182 antibody produced in rabbit has been used as a primary antibody for immunostaining of HeLa cells and human hepatoma cells. It has also been used for indirect immunofluorescence at a working concentration of 2.5-5.0μg/mL using human epithelial HEp-2 cells. |
| Biochem/physiol Actions: | Glycine-tryptophan (GW) bodies or P-bodies are cytoplasmic bodies that are involved in mRNA degradation, storage and translational repression. These bodies contain Argonaute proteins and m-RNAs that associate with GW182. GW182 is involved in post-transcriptional gene silencing/RNA interference (RNAi) via the microRNA pathway. Inhibition of gene expression leads to destruction of GWBs and disruption of RNAi and microRNA-induced gene silencing as it forms an integral component of GWBs. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | GW182 is a phosphorylated cytoplasmic autoantigen. It contains multiple glycine-tryptophan (GW) repeats along with a single RNA recognition motif. GW182 is a protein of 182 kDa, found in mammalian cell types. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |