Anti-G3BP antibody produced in rabbit
SIGMA/G6046 - ~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-G3BP1; Anti-GAP SH3 domain-binding protein; Anti-HDH-VIII; Anti-
Product Type: Chemical
Immunofluorescence HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Rabbit Anti-G3BP (Cat. No. G6046). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
Immunoblotting Whole extract of K562 cells was separated on SDS-PAGE and probed with Rabbit Anti-G3BP (Cat. No. G6046). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 0.1 μg/mL antibody 2. 0.2 μg/mL antibody 3. 0.2 μg/mL antibody + G3BP immunizing peptide (human, 230-247)
Immunoblotting Whole extract of U2OS cells was separated on SDS-PAGE and probed with Rabbit Anti-G3BP (Cat. No. G6046). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Lanes 1. 0.1 μg/mL antibody 2. 0.2 μg/mL antibody 3. 0.2 μg/mL antibody + G3BP immunizing peptide (human, 230-247)
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen ~68 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | human, mouse (predicted), rat (predicted) |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | indirect immunofluorescence: 2-5 μg/mL using human HeLa cells |
| western blot: 0.1-0.2 μg/mL using whole extract of human U-2-OS cells | |
| UniProt accession no. | Q13283  |
| Application: | Anti-G3BP antibody produced in rabbit has been used in co-immunoprecipitation assay. |
| Application: | Anti-G3BP antibody produced in rabbit has been used in • immunofluorescence analysis • immunostaining • immunoblotting • immunoprecipitation |
| Biochem/physiol Actions: | G3BP (Ras-GTPase-activating protein SH3 domain binding protein 1) is a endoribonuclease that is phosphorylation-dependent and specific to single strand. It cleaves between cytosine and adenine (CA). It binds to the SH3 domain of RasGAP. It forms a link between RasGAP-mediated signaling pathway and RNA turnover. G3BP is required for the assembly of stress granules (SGs), which are involved in the regulation of mRNA metabolism during stress. It may be used as a cytoplasmic SG marker. |
| Biochem/physiol Actions: | Ras-GAP SH3 domain binding protein (G3BP) is overexpressed in many kinds of malignant tumors such as breast, colon, lung and gastric breast cancer. G3BP expression is correlated with lymph node metastasis in breast cancer. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | G3BP encodes one of the DNA-unwinding enzymes which prefers partially unwound 3′-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. |
| General description: | G3BP is a RNA-binding protein that contains a RNA binding domain, the RRM-type domain, at the carboxyl-terminal. The N-terminal domain is homologous to nuclear transporter factor 2 (NTF2) and the central domain is rich in acidic residues. The RRM domain is involved in the binding of G3BP to specific RNA sequences. This helps G3BP to function as a CA dinucleotide-specific endoribonuclease. G3BP′s subcellular localization and protein-protein interactions are mediated by the phosphorylation of G3BP at Ser149, near NTF2-like domain. G3BP is found in abundance in many kinds of malignant tumors such as lung, colon, gastric, and breast cancer. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| Preparation Note: | In immunoblotting assays, it is highly recommended to dilute the antibody in 5% NFDM (w/v). |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |