Glycerokinase from Cellulomonas sp.
SIGMA/G6142 - lyophilized powder, 25-75 units/mg protein
Synonym: glpK; ATP:glycerol 3-phosphotransferase; Glycerol Kinase
CAS Number: 9030-66-4
EC Number: 232-862-0
MDL Number: MFCD00131205
Product Type: Chemical
Figure 1. Stability (powder form); kept under dry conditions
This picture is provided solely for illustration purposes. Optical properties of the actual product may deviate. Relevant product information is printed on labeled products and other accompanying or available information material. This image depicts SKU: G6142-1KU.
Figure 2. Stability (liquid form at 37 °C); buffer composition: 50 mM K-phosphate buffer, containing 3.2 M ammonium sulfate, pH 7.5
Figure 3. pH Activity; 25 °C; G-3-PDH system (○, 0.18 M glycine-hydrazine buffer); G-3-P oxidase system (●, 50 mM Tris-HCl buffer; △, 50 mM (2-N-Morpholino) ethanesulfonic acid-NaOH buffer)
Figure 4. Temperature activity; in 0.18 M glycine-hyddrazine buffer, pH 9.8
Figure 5. pH Stability; 25 °C, 20-hr treatment with 50 mM buffer solution: pH 4.0–6.0, acetate; pH 6.0–0.0 K-phosphate; pH 9.0–11.0, K2CO3-NaHCO3
Figure 6. Thermal stability; 15-min. treatment with 50 mM K-phosphate buffer, pH 7.5
| composition | Protein, ≥60% biuret |
| form | lyophilized powder |
| mol wt | ~128 kDa (by gel filtration) |
| Quality Level | 200  |
| specific activity | 25-75 units/mg protein |
| storage temp. | −20°C |
| Application: | Glycerokinase from Cellulomonas sp. has been used: • for determining the kinetic characteristics of human and trypanosomatid phosphofructokinases using an enzyme-linked kinetic assay. • to study the effect of sugar in fluorescence emission. • in 2-Arachidonoylglycerol-ba |
| Biochem/physiol Actions: | Glycerol kinase catalyzes the MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate |
| General description: | Research area: Cell Signaling Glycerol kinase (GK) is part of the FGGY carbohydrate kinase family. |
| General description: | Isoelectric point : 4.2 Michaelis constants : 4.4 x 10-5M (Glycerol), 4.3 x 10-4M (ATP) Inhibitors : p-Chloromercuribenzoate, heavy metal ions (Pb++, Fe++, Hg++, Ag+) Optimum pH : 9.8 (G-3-PDH system), 7.8 (G-3-P oxidase system) Optimum temperature : 500C pH Stability : pH 5.5 x 10.0 (25oC, 20hr) Thermal stability : below 40oC (pH 7.5, 15min) Substrate specificity : This enzyme catalyzes the stereospecific transfer of the terminal phosphoryl moiety of ATP to one of the primary hydroxyl group of glycerol, forming sn-glycerol-3-P. The enzyme has the highest specificity for glycerol, and also phosphorylates dihydroxyacetone and glyceraldehyde (Table 1,2). Mg++ is essentially required for the reaction. |
| Other Notes: | One unit will convert 1.0 μmole of glycerol and ATP to |
| Packaging: | 1000 units in poly bottle |
| Physical form: | Lyophilized powder containing phosphate buffer salts and sodium gluconate |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| activity | specific activity: 25-75 units/mg protein |
| Storage Temp. | −20°C |
| Enzyme Commission (EC) Number | 2.7.1.30 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |