Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal
SIGMA/G6171 - clone G-A-5, purified from hybridoma cell culture
Synonym: Anti-GFAP
MDL Number: MFCD00145904
Product Type: Chemical
Immunohistochemistry-immunofluorescence JOURNAL CITATION: Unidirectional photoreceptor-to-Müller glia coupling and unique K+ channel expression in Caiman retina. By: Zayas-Santiago, A., Agte, S., et al. in PLoS One, 2014. PubMed ID: 24831221 Image collected and cropped by CiteAb from the following publication, (http://dx.plos.org/10.1371/journal.pone.0097155), provided under a CC-BY license. Image might reflect a new usage that is not reflected in claims on product description or independently verified by Merck KGaA, Darmstadt, Germany. For Research Use Only. Not for use in diagnostic procedures.
Immunofluorescence Rat cerebellum sections ethanol fixed and paraffin embedded were stained with 2.5-5 μg /mL of Monoclonal Anti-GFAP (Cat. No. G6171). The antibody was developed with Goat Anti-Mouse IgG, FITC conjugate (Cat. No. F8771).
Immunohistochemistry Staining of ethanol fixed, paraffin embedded Rat cerebellum sections with 5 μg/mL Monoclonal Anti-Glial Fibrillary Acidic Protein, Clone: G-A-5 (Cat. No. G6171). The antibody was developed with Goat Anti-Mouse IgG, FITC conjugate (Cat. No. F8771).
| antibody form | purified from hybridoma cell culture |
| purified immunoglobulin | |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | G-A-5, monoclonal |
| concentration | ~1.0 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| isotype | IgG1 |
| mol wt | antigen ~50 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | rat, human, pig |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunocytochemistry: suitable |
| immunohistochemistry: suitable | |
| indirect immunofluorescence: 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum. | |
| western blot: suitable | |
| UniProt accession no. | P14136  |
| Application: | Anti-Glial Fibrillary Acidic Protein antibody, Mouse monoclonal has been used in immunocytochemistry and in immunofluorescence. |
| Application: | Monoclonal Anti-Glial Fibrillary Acidic Protein antibody is suitable for use in western blot and indirect immunofluorescence 2.5-5 μg/mL using alcohol-fixed sections of rat brain/cerebellum). The antibody can also be used for immunoblot (approx. 50 kDa), immunohistochemistry (in paraformaldehyde-fixed, frozen rabbit brain sections) and immunocytochemistry. |
| Biochem/physiol Actions: | Glial fibrillary acidic protein or GFAP assembly has been implicated in mitotic remodeling of glial structures. Genetic mutations in GFAP have been associated with Alexander disease. |
| Biochem/physiol Actions: | Microdeletion in glial fibrillary acidic protein (GFAP) gene locus is implicated in intellectual disability and facial dysmorphism in children. High levels of GFAP is associated with acute intracerebral haemorrhage. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Glial fibrillary acidic protein (GFAP) is intermediary filament present in astrocyte cells and is expressed in 10 different isoforms. It comprises of N-terminal head, central rod and C-terminal tail domains. GFAP is mapped to human chromosome 17q21.31. |
| Immunogen: | purified GFAP from pig spinal cord. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |