Glycerokinase from Escherichia coli
SIGMA/G6278 - 300-600 units/mL, ammonium sulfate suspension
Synonym: ATP:glycerol 3-phosphotransferase; Glycerol Kinase
CAS Number: 9030-66-4
EC Number: 232-862-0
MDL Number: MFCD00066766
Product Type: Chemical
Unit Definition One unit will convert 1.0 μmole of glycerol and ATP to L-α-glycerophosphate and ADP per min at pH 9.8 at 25°C in a coupled system with PK/LDH.
| biological source | Escherichia coli |
| concentration | 300-600 units/mL |
| form | ammonium sulfate suspension |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | Glycerol kinase (glpK) was used to study the effects of pain controlling neuropeptides on human fat cell lipolysis. |
| Application: | Glycerol kinase has been used along with adenosine triphosphate (ATP) for the conversion of glycerol to glycerol-3 phosphate (glycerol-3P) in white adipose tissue (WAT) adipocyte samples by label distribution method. It has also been used along with adenosine triphosphate (ATP) in the derivatization of glycerol to sn-glycerol-3 phosphate (glycerol-3P). |
| Biochem/physiol Actions: | Glycerol kinase (GK), the primary enzyme involved in regulating glycerol uptake and its metabolism, was initially identified in E. coli. This enzyme acts as a catalyst in the Mg2+-ATP-dependent phosphorylation of glycerol to produce G3P, a crucial intermediate in various metabolic pathways, including the synthesis of glycerolipids and triglycerides, as well as glycogenesis, glycolysis, and gluconeogenesis. GK participates in the glycerol 3-phosphate shuttle and, in conjunction with glycerol 3-phosphate dehydrogenase, converts glycerol into dihydroxyacetone phosphate. It plays a pivotal role in plasma glycerol withdrawal, glycerol utilization by different tissues, and in maintaining carbohydrate homeostasis. GK is positioned at the nexus of lipid and carbohydrate metabolism and may play a potential role in type 2 diabetes mellitus (T2DM). |
| Biochem/physiol Actions: | Glycerol kinase catalyzes tge MgATP-dependent phosphorylation of glycerol to produce sn-glycerol-3-phosphate and is the rate limiting enzyme in the utilization of glycerol. It is also subject to feedback regulation by fructose-1,6-bisphosphate |
| General description: | Research Area: Cell Signaling Glycerol kinase (GK) is a member of the FGGY carbohydrate kinase family (ATP: glycerol 3-phosphotransferase), which is responsible for transferring a phosphate group from ATP to glycerol. |
| Other Notes: | One unit will convert 1.0 μmole of glycerol and ATP to |
| Physical form: | Suspension in 3.1 M (NH4)2SO4 pH 7.3, with 1% BSA and 2% trehalose |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| Enzyme Commission (EC) Number | 2.7.1.30 ( BRENDA  | IUBMB ) |
| UNSPSC | 12352204 |