Monoclonal Anti-β-Galactosidase antibody produced in mouse
SIGMA/G6282 - clone GAL-40, ascites fluid
Synonym: Mouse Anti-
MDL Number: MFCD00162155
Product Type: Chemical
Immunoblotting Extract of E. coli overexpressing β-Galactosidase was separated on SDS-PAGE and probed with Monoclonal Anti-β-Galactosidase Clone: GAL-40 (Cat. No. G6282). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate. Lanes 1. Antibody dilution 1:2,000 2. Negative Control: without 1st antibody
| antibody form | ascites fluid |
| antibody product type | primary antibodies |
| biological source | mouse |
| clone | GAL-40, monoclonal |
| conjugate | unconjugated |
| contains | 15 mM sodium azide |
| isotype | IgM |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | bacteria |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | dot blot: suitable using soluble β-galactosidase |
| immunocytochemistry: suitable | |
| western blot: 1:1,000 using denatured-reduced Escherichia coli β-galactosidase |
| Application: | Monoclonal anti-β-galactosidase antibodies may be used in dot blot (using soluble β-galactosidase), western blot (at a dilution of 1:1,000, using denatured-reduced Escherichia coli β-galactosidase) and immunocytochemistry. This antibody may also be used for immunochemical assays such as ELISA, immunoblot and immunohistochemistry by using the stepwise procedure or by the preparation of β-galactosidase anti-β galactosidase (BBAG) complex. Additionally, this product is suitable for the detection and purification of recombinant fusion proteins encoded by cloning vectors containing the β-galactosidase gene. Monoclonal anti-β-galactosidase antibody is also used for the immunoenzymatic staining of bone marrow and blood smears, and tissue sections. |
| Application: | Monoclonal Anti-β-Galactosidase antibody produced in mouse has been used for western blotting. |
| Biochem/physiol Actions: | β-galactosidase catalyses the hydrolysis of lactose to form glucose and galactose. It serves as a reporter enzyme in a number of analysis such as studying the regulation of gene expression, protein function and structure determination, and examining of target gene expression. β-galactosidase is also known to yield coloured products by acting on several substrates. Thus, it is prefered as a reporter enzyme in applications involving gene expression regulation, analysis of protein function/structure, and target gene expression. |
| Biochem/physiol Actions: | The antibody may be used for detection of β-galactosidase expressed by the E. coli lacZ gene encoded in many cloned gene sequences; serves as an indicator for fusion proteins encoded by an inserted DNA sequence. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | β-galactosidase is a ~465 kDa tetrameric protein. Eukaryotic genes are usually cloned into E.coli β-galactosidase (lacZ) gene that results in a fusion hybrid of the desired gene product with the β-galactosidase enzyme . As the amount of the desired gene product in these cloned vector systems corresponds to the amount of β-galactosidase, enzyme assays for β-galactosidase can be utilized to detect the required gene product . Monoclonal anti-β-galactosidase antibodies are specific for β-galactosidase in E.coli and enable the analysis of β-galactosidase fusion proteins encoded by cloned vector systems. |
| General description: | β-Galactosidase is coded by the LacZ gene which helps to transform lactose into galactose. |
| Immunogen: | β- |
| Hazard Codes | Xn |
| Risk Statements | 21/22 |
| Safety Statements | 36/37-60 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |